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| <SX load='6of4' size='340' side='right' viewer='molstar' caption='[[6of4]], [[Resolution|resolution]] 3.20Å' scene=''> | | <SX load='6of4' size='340' side='right' viewer='molstar' caption='[[6of4]], [[Resolution|resolution]] 3.20Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6of4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OF4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OF4 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6of4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OF4 FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0066080 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD]), CTHT_0016120 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6of4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6of4 OCA], [http://pdbe.org/6of4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6of4 RCSB], [http://www.ebi.ac.uk/pdbsum/6of4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6of4 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6of4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6of4 OCA], [https://pdbe.org/6of4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6of4 RCSB], [https://www.ebi.ac.uk/pdbsum/6of4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6of4 ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/G0SGE9_CHATD G0SGE9_CHATD] |
| Ribosome assembly is a complex process reliant on the coordination of trans-acting enzymes to produce functional ribosomal subunits and secure the translational capacity of cells. The endoribonuclease (RNase) Las1 and the polynucleotide kinase (PNK) Grc3 assemble into a multienzyme complex, herein designated RNase PNK, to orchestrate processing of precursor ribosomal RNA (rRNA). RNase PNK belongs to the functionally diverse HEPN nuclease superfamily, whose members rely on distinct cues for nuclease activation. To establish how RNase PNK coordinates its dual enzymatic activities, we solved a series of cryo-EM structures of Chaetomium thermophilum RNase PNK in multiple conformational states. The structures reveal that RNase PNK adopts a butterfly-like architecture, harboring a composite HEPN nuclease active site flanked by discrete RNA kinase sites. We identify two molecular switches that coordinate nuclease and kinase function. Together, our structures and corresponding functional studies establish a new mechanism of HEPN nuclease activation essential for ribosome production.
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| Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex.,Pillon MC, Hsu AL, Krahn JM, Williams JG, Goslen KH, Sobhany M, Borgnia MJ, Stanley RE Nat Struct Mol Biol. 2019 Sep;26(9):830-839. doi: 10.1038/s41594-019-0289-8. Epub, 2019 Sep 5. PMID:31488907<ref>PMID:31488907</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6of4" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] | | *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] |
| == References ==
| |
| <references/>
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| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: Chatd]] | | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Borgnia, M J]] | | [[Category: Borgnia MJ]] |
| [[Category: Goslen, K H]] | | [[Category: Goslen KH]] |
| [[Category: Hsu, A L]] | | [[Category: Hsu AL]] |
| [[Category: Krahn, J M]] | | [[Category: Krahn JM]] |
| [[Category: Pillon, M C]] | | [[Category: Pillon MC]] |
| [[Category: Sobhany, M]] | | [[Category: Sobhany M]] |
| [[Category: Stanley, R E]] | | [[Category: Stanley RE]] |
| [[Category: Williams, J G]] | | [[Category: Williams JG]] |
| [[Category: Complex]]
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| [[Category: Polynucleotide kinase]]
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| [[Category: Ribonuclease]]
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| [[Category: Rna binding protein]]
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