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| <SX load='6nut' size='340' side='right' viewer='molstar' caption='[[6nut]], [[Resolution|resolution]] 3.10Å' scene=''> | | <SX load='6nut' size='340' side='right' viewer='molstar' caption='[[6nut]], [[Resolution|resolution]] 3.10Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6nut]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebov-may Ebov-may] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NUT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NUT FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6nut]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebola_virus_-_Mayinga,_Zaire,_1976 Ebola virus - Mayinga, Zaire, 1976] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NUT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NUT FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=128952 EBOV-May])</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nut FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nut OCA], [http://pdbe.org/6nut PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nut RCSB], [http://www.ebi.ac.uk/pdbsum/6nut PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nut ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nut FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nut OCA], [https://pdbe.org/6nut PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nut RCSB], [https://www.ebi.ac.uk/pdbsum/6nut PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nut ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/NCAP_EBOZM NCAP_EBOZM]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases. | | [https://www.uniprot.org/uniprot/NCAP_EBOZM NCAP_EBOZM] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases. |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Ebola virus is an emerging virus that is capable of causing a deadly disease in humans. Replication, transcription and packaging of the viral genome are carried out by the viral nucleocapsid. The nucleocapsid is a complex of the viral nucleoprotein, RNA and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins. The 3.1 A resolution single-particle cryo-electron microscopy structure of the nucleoprotein-RNA helical filament presented here resembles previous structures determined at lower resolution, while providing improved molecular details of protein-protein and protein-RNA interactions. The higher resolution of the structure presented here will facilitate the design and characterization of novel and specific Ebola virus therapeutics targeting the nucleocapsid.
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| Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex.,Kirchdoerfer RN, Saphire EO, Ward AB Acta Crystallogr F Struct Biol Commun. 2019 May 1;75(Pt 5):340-347. doi:, 10.1107/S2053230X19004424. Epub 2019 Apr 24. PMID:31045563<ref>PMID:31045563</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6nut" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: Ebov-may]] | | [[Category: Ebola virus - Mayinga, Zaire, 1976]] |
| [[Category: Homo sapiens]] | | [[Category: Homo sapiens]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Kirchdoerfer, R N]] | | [[Category: Kirchdoerfer RN]] |
| [[Category: Ward, A B]] | | [[Category: Ward AB]] |
| [[Category: Capsid]]
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| [[Category: Nucleocapsid]]
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| [[Category: Nucleoprotein]]
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| [[Category: Rna-binding]]
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| [[Category: Viral protein-rna complex]]
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