4ijs: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4ijs]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bunyamwera_virus Bunyamwera virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IJS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IJS FirstGlance]. <br>
<table><tr><td colspan='2'>[[4ijs]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bunyamwera_virus Bunyamwera virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IJS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IJS FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ijs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ijs OCA], [https://pdbe.org/4ijs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ijs RCSB], [https://www.ebi.ac.uk/pdbsum/4ijs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ijs ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ijs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ijs OCA], [https://pdbe.org/4ijs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ijs RCSB], [https://www.ebi.ac.uk/pdbsum/4ijs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ijs ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/NCAP_BUNYW NCAP_BUNYW]  
[https://www.uniprot.org/uniprot/NCAP_BUNYW NCAP_BUNYW]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bunyamwera virus (BUNV), which belongs to the genus Orthobunyavirus, is the prototypical virus of the Bunyaviridae family. Similar to other negative-sense single-stranded RNA viruses, bunyaviruses possess a nucleocapsid protein (NP) to facilitate genomic RNA encapsidation and virus replication. The structures of two NPs of members of different genera within the Bunyaviridae family have been reported. However, their structures, RNA-binding features, and functions beyond RNA binding significantly differ from one another. Here, we report the crystal structure of the BUNV NP-RNA complex. The polypeptide of the BUNV NP was found to possess a distinct fold among viral NPs. An N-terminal arm and a C-terminal tail were found to interact with neighboring NP protomers to form a tetrameric ring-shaped organization. Each protomer bound a 10-nt RNA molecule, which was acquired from the expression host, in the positively charged crevice between the N and C lobes. Inhomogeneous oligomerization was observed for the recombinant BUNV NP-RNA complex, which was similar to the Rift Valley fever virus NP-RNA complex. This result suggested that the flexibility of one NP protomer with adjacent protomers underlies the BUNV ribonucleoprotein complex (RNP) formation. Electron microscopy revealed that the monomer-sized NP-RNA complex was the building block of the natural BUNV RNP. Combined with previous results indicating that mutagenesis of the interprotomer or protein-RNA interface affects BUNV replication, our structure provides a great potential for understanding the mechanism underlying negative-sense single-stranded RNA RNP formation and enables the development of antiviral therapies targeting BUNV RNP formation.
Bunyamwera virus possesses a distinct nucleocapsid protein to facilitate genome encapsidation.,Li B, Wang Q, Pan X, Fernandez de Castro I, Sun Y, Guo Y, Tao X, Risco C, Sui SF, Lou Z Proc Natl Acad Sci U S A. 2013 Apr 8. PMID:23569257<ref>PMID:23569257</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ijs" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>

Latest revision as of 11:53, 20 March 2024

Crystal structure of nucleocapsid protein encoded by the prototypic member of orthobunyavirusCrystal structure of nucleocapsid protein encoded by the prototypic member of orthobunyavirus

Structural highlights

4ijs is a 8 chain structure with sequence from Bunyamwera virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NCAP_BUNYW

See Also

4ijs, resolution 3.20Å

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