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==Crystal structure of Helicobacter pylori MinE, a cell division topological specificity factor==
==Crystal structure of Helicobacter pylori MinE, a cell division topological specificity factor==
<StructureSection load='3mcd' size='340' side='right' caption='[[3mcd]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<StructureSection load='3mcd' size='340' side='right'caption='[[3mcd]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3mcd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43504 Atcc 43504]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MCD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MCD FirstGlance]. <br>
<table><tr><td colspan='2'>[[3mcd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MCD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MCD FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ku7|3ku7]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">minE, HP_0332 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=210 ATCC 43504])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mcd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mcd OCA], [https://pdbe.org/3mcd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mcd RCSB], [https://www.ebi.ac.uk/pdbsum/3mcd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mcd ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mcd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mcd OCA], [http://pdbe.org/3mcd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mcd RCSB], [http://www.ebi.ac.uk/pdbsum/3mcd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mcd ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/MINE_HELPY MINE_HELPY]] Prevents the cell division inhibition by proteins MinC and MinD at internal division sites while permitting inhibition at polar sites. This ensures cell division at the proper site by restricting the formation of a division septum at the midpoint of the long axis of the cell (By similarity).  
[https://www.uniprot.org/uniprot/MINE_HELPY MINE_HELPY] Prevents the cell division inhibition by proteins MinC and MinD at internal division sites while permitting inhibition at polar sites. This ensures cell division at the proper site by restricting the formation of a division septum at the midpoint of the long axis of the cell (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mcd ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mcd ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In Gram-negative bacteria, proper placement of the FtsZ ring, mediated by nucleoid occlusion and the activities of the dynamic oscillating Min proteins MinC, MinD and MinE, is required for correct positioning of the cell division septum. MinE is a topological specificity factor that counters the activity of MinCD division inhibitor at the mid-cell division site. Its structure consists of an anti-MinCD domain and a topology specificity domain (TSD). Previous NMR analysis of truncated Escherichia coli MinE showed that the TSD domain contains a long alpha-helix and two anti-parallel beta-strands, which mediate formation of a homodimeric alpha/beta structure. Here we report the crystal structure of full-length Helicobacter pylori MinE and redefine its TSD based on that structure. The N-terminal region of the TSD (residues 19-26), previously defined as part of the anti-MinCD domain, forms a beta-strand (betaA) and participates in TSD folding. In addition, H. pylori MinE forms a dimer through the interaction of anti-parallel betaA-strands. Moreover, we observed serial dimer-dimer interactions within the crystal packing, resulting in the formation of a multimeric structure. We therefore redefine the functional domain of MinE and propose that a multimeric filamentous structure is formed through anti-parallel beta-strand interactions.
Crystal structure of Helicobacter pylori MinE, a cell division topological specificity factor.,Kang GB, Song HE, Kim MK, Youn HS, Lee JG, An JY, Chun JS, Jeon H, Eom SH Mol Microbiol. 2010 Jun 1;76(5):1222-31. Epub 2010 Apr 14. PMID:20398219<ref>PMID:20398219</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3mcd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 43504]]
[[Category: Helicobacter pylori]]
[[Category: An, J Y]]
[[Category: Large Structures]]
[[Category: Chun, J S]]
[[Category: An JY]]
[[Category: Eom, S H]]
[[Category: Chun JS]]
[[Category: Jeon, H]]
[[Category: Eom SH]]
[[Category: Kang, G B]]
[[Category: Jeon H]]
[[Category: Kim, M K]]
[[Category: Kang GB]]
[[Category: Lee, J G]]
[[Category: Kim MK]]
[[Category: Song, H E]]
[[Category: Lee JG]]
[[Category: Youn, H S]]
[[Category: Song HE]]
[[Category: Cell cycle]]
[[Category: Youn HS]]
[[Category: Cell division]]
[[Category: Cytokinesis]]
[[Category: Mine]]
[[Category: Topological specificity factor]]

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