3is1: Difference between revisions

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<StructureSection load='3is1' size='340' side='right'caption='[[3is1]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
<StructureSection load='3is1' size='340' side='right'caption='[[3is1]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3is1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stas1 Stas1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IS1 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3is1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_saprophyticus_subsp._saprophyticus_ATCC_15305_=_NCTC_7292 Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IS1 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3irp|3irp]], [[3irz|3irz]], [[3is0|3is0]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSP0135, uafA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342451 STAS1])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3is1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3is1 OCA], [https://pdbe.org/3is1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3is1 RCSB], [https://www.ebi.ac.uk/pdbsum/3is1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3is1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3is1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3is1 OCA], [https://pdbe.org/3is1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3is1 RCSB], [https://www.ebi.ac.uk/pdbsum/3is1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3is1 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/UAFA_STAS1 UAFA_STAS1]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3is1 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3is1 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Staphylococci use cell wall-anchored proteins as adhesins to attach to host tissues. Staphylococcus saprophyticus, a uropathogenic species, has a unique cell wall-anchored protein, uro-adherence factor A (UafA), which shows erythrocyte binding activity. To investigate the mechanism of adhesion by UafA, we determined the crystal structure of the functional region of UafA at 1.5 A resolution. The structure was composed of three domains, designated as the N2, N3, and B domains, arranged in a triangular relative configuration. Hemagglutination inhibition assay with domain-truncated mutants indicated that both N and B domains were necessary for erythrocyte binding. Based on these results, a novel manner of ligand binding in which the B domain acts as a functional domain was proposed as the adhesion mechanism of S. saprophyticus.
Crystal structure of the functional region of Uro-adherence factor A from Staphylococcus saprophyticus reveals participation of the B domain in ligand binding.,Matsuoka E, Tanaka Y, Kuroda M, Shouji Y, Ohta T, Tanaka I, Yao M Protein Sci. 2011 Feb;20(2):406-16. doi: 10.1002/pro.573. PMID:21280131<ref>PMID:21280131</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3is1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Stas1]]
[[Category: Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292]]
[[Category: Kuroda, M]]
[[Category: Kuroda M]]
[[Category: Matsuoka, E]]
[[Category: Matsuoka E]]
[[Category: Shouji, Y]]
[[Category: Shouji Y]]
[[Category: Tanaka, I]]
[[Category: Tanaka I]]
[[Category: Tanaka, Y]]
[[Category: Tanaka Y]]
[[Category: Yao, M]]
[[Category: Yao M]]
[[Category: Cell adhesion]]
[[Category: Cell wall]]
[[Category: Dev-igg fold]]
[[Category: Hemagglutinin]]
[[Category: Peptidoglycan-anchor]]
[[Category: Secreted]]
[[Category: Virulence]]

Latest revision as of 11:28, 20 March 2024

Crystal structure of functional region of UafA from Staphylococcus saprophyticus in C2 form at 2.45 angstrom resolutionCrystal structure of functional region of UafA from Staphylococcus saprophyticus in C2 form at 2.45 angstrom resolution

Structural highlights

3is1 is a 1 chain structure with sequence from Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.45Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

UAFA_STAS1

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

3is1, resolution 2.45Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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