UvrABC: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
UvrA contains several domains: <scene name='53/536710/Cv/2'>UvrB-binding, DNA-binding and two ATP-binding domains</scene><ref>PMID:21240268</ref>.
UvrA contains several domains: <scene name='53/536710/Cv/2'>UvrB-binding, DNA-binding and two ATP-binding domains</scene><ref>PMID:21240268</ref>.
</StructureSection>


==3D structures of UvrABC==
==3D structures of UvrABC==


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
[[UvrABC 3D structures]]
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*UvrA


**[[3pih]] – TmUvrA + DNA – ''Thermotoga maritima'' <br />
**[[6n9l]] - TmUvrA (mutant) + ADP<br />
**[[3zqj]] – UvrA  – ''Mycobacterium tuberculosis''<br />
**[[4dfc]] – EcUvrA + Transcription-repair-coupling factor – ''Escherichia coli'' <br />
**[[2r6f]] – BstUvrA – ''Bacillus stearothermophilus'' <br />
**[[3ux8]] – GeUvrA - ''Geobacillus''<br />
**[[2vf7]], [[2vf8]] – DrUvrA2 - ''Deinococcus radiodurans''<br />
*UvrB
**[[1d2m]], [[1c4o]] – TtUvrB – ''Thermus thermophilus'' <br />
**[[1d9x]] – BcUvrB – ''Bacillus caldotenax'' <br />
**[[1t5l]] – BcUvrB (mutant) <br />
**[[1qoj]] – EcUvrB C terminal <br />
**[[1e52]] – EcUvrB C terminal - NMR<br />
**[[1d9z]] – BcUvrB + ATP <br />
**[[2fdc]], [[6o8f]], [[6o8g]], [[6o8h]] – BcUvrB + DNA <br />
**[[6o8e]] – BcUvrB + DNA + ADP<br />
**[[2d7d]], [[2nmv]], [[3v4r]] – BsUvrB + DNA – ''Bacillus subtilis'' <br />
*UvrC
**[[1kft]], [[1qoj]] – EcUvrC C terminal <br />
**[[1e52]] – EcUvrC C terminal - NMR<br />
**[[2nrr]], [[2nrt]], [[2nrv]], [[2nrw]], [[2nrx]], [[2nrz]] – TmUvrC C terminal <br />
**[[1ycz]], [[1yd0]], [[1yd1]] – TmUvrC N terminal <br />
**[[1yd2]], [[1yd3]], [[1yd4]], [[1yd5]] – TmUvrC N terminal (mutant)<br />
**[[1yd6]] – BcUvrC N terminal <br />
**[[3c65]] – BstUvrC  <br />
*UvrD or DNA helicase II see [[Helicase]]
*UvrA-UvrB
**[[3fpn]] – BstUvrA + UvrB <br />
**[[3uwx]] – GeUvrA + UvrB <br />
*UvrB-UvrD
**[[7egt]] – TtUvrB N-terminal + UvrD C-terminal <br />
}}
==References==
==References==
<references />
<references />
</StructureSection>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 10:52, 17 March 2024

Function

UvrABC endonuclease is an E. coli enzyme complex involved in DNA repair. UvrABC removes 12 nucleotides around a DNA mutation replacing them with the correct one[1].

  • UvrA is the protein which locates the DNA damage[2].
  • UvrB is involved in distinguishing damaged from undamaged DNA[3].
  • The C-terminal region of UvrC is involved in DNA binding and incisions at the 5'-side of a DNA damage during nucleotide excision repair[4].
  • UvrD is DNA helicase II. See Helicase.

For details on the UvrA-UvrB complex see UvrA-UvrB interaction domains.

Structural highlights

UvrA contains several domains: [5].

3D structures of UvrABC

UvrABC 3D structures

References

  1. ↑ Moolenaar GF, Moorman C, Goosen N. Role of the Escherichia coli nucleotide excision repair proteins in DNA replication. J Bacteriol. 2000 Oct;182(20):5706-14. PMID:11004168
  2. ↑ Jaciuk M, Nowak E, Skowronek K, Tanska A, Nowotny M. Structure of UvrA nucleotide excision repair protein in complex with modified DNA. Nat Struct Mol Biol. 2011 Feb;18(2):191-7. Epub 2011 Jan 16. PMID:21240268 doi:10.1038/nsmb.1973
  3. ↑ Theis K, Skorvaga M, Machius M, Nakagawa N, Van Houten B, Kisker C. The nucleotide excision repair protein UvrB, a helicase-like enzyme with a catch. Mutat Res. 2000 Aug 30;460(3-4):277-300. PMID:10946234
  4. ↑ Moolenaar GF, Uiterkamp RS, Zwijnenburg DA, Goosen N. The C-terminal region of the Escherichia coli UvrC protein, which is homologous to the C-terminal region of the human ERCC1 protein, is involved in DNA binding and 5'-incision. Nucleic Acids Res. 1998 Jan 15;26(2):462-8. PMID:9421501
  5. ↑ Jaciuk M, Nowak E, Skowronek K, Tanska A, Nowotny M. Structure of UvrA nucleotide excision repair protein in complex with modified DNA. Nat Struct Mol Biol. 2011 Feb;18(2):191-7. Epub 2011 Jan 16. PMID:21240268 doi:10.1038/nsmb.1973

UvrA complex with DNA, pyrophosphate and Zn+2 ion (grey) 3pih

Drag the structure with the mouse to rotate

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Michal Harel, Alexander Berchansky
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