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| <StructureSection load='3v3s' size='340' side='right'caption='[[3v3s]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='3v3s' size='340' side='right'caption='[[3v3s]], [[Resolution|resolution]] 1.90Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3v3s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V3S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V3S FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3v3s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V3S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V3S FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2qpn|2qpn]]</div></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">blaGes ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
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| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v3s OCA], [https://pdbe.org/3v3s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v3s RCSB], [https://www.ebi.ac.uk/pdbsum/3v3s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v3s ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v3s OCA], [https://pdbe.org/3v3s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v3s RCSB], [https://www.ebi.ac.uk/pdbsum/3v3s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v3s ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/H2ERK4_PSEAI H2ERK4_PSEAI] |
| A clinical isolate of Pseudomonas aeruginosa recovered from the lower respiratory tract of an 81-year old patient hospitalized in Belgium was sent to the national reference center to determine its resistance mechanism. PCR-sequencing identified a new GES variant, GES-18, which differs from the carbapenem-hydrolyzing enzyme GES-5 by a single amino acid substitution (Val80Ile, numbering according to Ambler) and from GES-1 by two substitutions (Val80Ile and Gly170Ser). Detailed kinetic characterization showed that GES-18 and GES-5 hydrolyze imipenem and cefoxitin with similar kinetic parameters and that GES-18 was less susceptible than GES-1 to classical beta-lactamase inhibitors such as clavulanate and tazobactam. The overall structure of GES-18 is similar to the solved structures of GES-1 and GES-2, the Val80Ile and Gly170Ser substitutions causing only subtle local rearrangements. Notably, the hydrolytic water molecule and the Glu166 residue were slightly displaced compared to their counterparts in GES-1. Our kinetic and crystallographic data for GES-18 highlight the pivotal role of the Gly170Ser substitution which distinguishes GES-5 and GES-18 from GES-1.
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| GES-18, a new carbapenem-hydrolyzing GES-type beta-lactamase from Pseudomonas aeruginosa that contains Ile80 and Ser170 residues.,Bebrone C, Bogaerts P, Delbruck H, Bennink S, Kupper MB, Rezende de Castro R, Glupczynski Y, Hoffmann KM Antimicrob Agents Chemother. 2012 Oct 31. PMID:23114760<ref>PMID:23114760</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3v3s" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]] | | *[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Beta-lactamase]]
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| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Bebrone, C]] | | [[Category: Pseudomonas aeruginosa]] |
| [[Category: Delbruck, H]] | | [[Category: Bebrone C]] |
| [[Category: Hoffmann, K M.V]] | | [[Category: Delbruck H]] |
| [[Category: Beta lactamase fold]] | | [[Category: Hoffmann KMV]] |
| [[Category: Beta lactam]]
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| [[Category: Hydrolase]]
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