3uq4: Difference between revisions

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<StructureSection load='3uq4' size='340' side='right'caption='[[3uq4]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
<StructureSection load='3uq4' size='340' side='right'caption='[[3uq4]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3uq4]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UQ4 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3uq4]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_dadantii_3937 Dickeya dadantii 3937]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UQ4 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3uq5|3uq5]], [[3uq7|3uq7]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq4 OCA], [https://pdbe.org/3uq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uq4 RCSB], [https://www.ebi.ac.uk/pdbsum/3uq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uq4 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq4 OCA], [https://pdbe.org/3uq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uq4 RCSB], [https://www.ebi.ac.uk/pdbsum/3uq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uq4 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/E0SJQ4_DICD3 E0SJQ4_DICD3]
The determination of structural models of the various stable states of an ion channel is a key step toward the characterization of its conformational dynamics. In the case of nicotinic-type receptors, different structures have been solved but, thus far, these different models have been obtained from different members of the superfamily. In the case of the bacterial member ELIC, a cysteamine-gated channel from Erwinia chrisanthemi, a structural model of the protein in the absence of activating ligand (and thus, conceivably corresponding to the closed state of this channel) has been previously generated. In this article, electrophysiological characterization of ELIC mutants allowed us to identify pore mutations that slow down the time course of desensitization to the extent that the channel seems not to desensitize at all for the duration of the agonist applications (&gt;20 min). Thus, it seems reasonable to conclude that the probability of ELIC occupying the closed state is much lower for the ligand-bound mutants than for the unliganded wild-type channel. To gain insight into the conformation adopted by ELIC under these conditions, we solved the crystal structures of two of these mutants in the presence of a concentration of cysteamine that elicits an intracluster open probability of &gt;0.9. Curiously, the obtained structural models turned out to be nearly indistinguishable from the model of the wild-type channel in the absence of bound agonist. Overall, our findings bring to light the limited power of functional studies in intact membranes when it comes to inferring the functional state of a channel in a crystal, at least in the case of the nicotinic-receptor superfamily.


Mutations that stabilize the open state of the Erwinia chrisanthemi ligand-gated ion channel fail to change the conformation of the pore domain in crystals.,Gonzalez-Gutierrez G, Lukk T, Agarwal V, Papke D, Nair SK, Grosman C Proc Natl Acad Sci U S A. 2012 Apr 2. PMID:22474383<ref>PMID:22474383</ref>
==See Also==
 
*[[Ion channels 3D structures|Ion channels 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3uq4" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Dicd3]]
[[Category: Dickeya dadantii 3937]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Agarwal, V]]
[[Category: Agarwal V]]
[[Category: Gonzalez-Gutierrez, G]]
[[Category: Gonzalez-Gutierrez G]]
[[Category: Grosman, C]]
[[Category: Grosman C]]
[[Category: Lukk, T]]
[[Category: Lukk T]]
[[Category: Nair, S K]]
[[Category: Nair SK]]
[[Category: Papke, D]]
[[Category: Papke D]]
[[Category: Liganded-gated ion channel]]
[[Category: Membrane protein]]
[[Category: Transport protein]]

Latest revision as of 17:05, 14 March 2024

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant F247L (F16L)X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant F247L (F16L)

Structural highlights

3uq4 is a 10 chain structure with sequence from Dickeya dadantii 3937. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.5Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

E0SJQ4_DICD3

See Also

3uq4, resolution 3.50Å

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