3tl6: Difference between revisions

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<StructureSection load='3tl6' size='340' side='right'caption='[[3tl6]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
<StructureSection load='3tl6' size='340' side='right'caption='[[3tl6]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3tl6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Enthi Enthi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TL6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TL6 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3tl6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TL6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TL6 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EHI_130960 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tl6 OCA], [https://pdbe.org/3tl6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tl6 RCSB], [https://www.ebi.ac.uk/pdbsum/3tl6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tl6 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tl6 OCA], [https://pdbe.org/3tl6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tl6 RCSB], [https://www.ebi.ac.uk/pdbsum/3tl6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tl6 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/C4LXG4_ENTH1 C4LXG4_ENTH1]
Despite recent advances, the expression of heterologous proteins in Escherichia coli for crystallization remains a nontrivial challenge. The present study investigates the efficacy of maltose-binding protein (MBP) fusion as a general strategy for rescuing the expression of target proteins. From a group of sequence-verified clones with undetectable levels of protein expression in an E. coli T7 expression system, 95 clones representing 16 phylogenetically diverse organisms were selected for recloning into a chimeric expression vector with an N-terminal histidine-tagged MBP. PCR-amplified inserts were annealed into an identical ligation-independent cloning region in an MBP-fusion vector and were analyzed for expression and solubility by high-throughput nickel-affinity binding. This approach yielded detectable expression of 72% of the clones; soluble expression was visible in 62%. However, the solubility of most proteins was marginal to poor upon cleavage of the MBP tag. This study offers large-scale evidence that MBP can improve the soluble expression of previously non-expressing proteins from a variety of eukaryotic and prokaryotic organisms. While the behavior of the cleaved proteins was disappointing, further refinements in MBP tagging may permit the more widespread use of MBP-fusion proteins in crystallographic studies.
 
Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.,Hewitt SN, Choi R, Kelley A, Crowther GJ, Napuli AJ, Van Voorhis WC Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt, 9):1006-9. Epub 2011 Aug 13. PMID:21904041<ref>PMID:21904041</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3tl6" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Purine nucleoside phosphorylase 3D structures|Purine nucleoside phosphorylase 3D structures]]
*[[Purine nucleoside phosphorylase 3D structures|Purine nucleoside phosphorylase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Enthi]]
[[Category: Entamoeba histolytica]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Purine-nucleoside phosphorylase]]
[[Category: Clifton MC]]
[[Category: Clifton, M C]]
[[Category: Edwards TE]]
[[Category: Edwards, T E]]
[[Category: Structural genomic]]
[[Category: Anaerobic parasitic protozoan]]
[[Category: Digestive tract cyst]]
[[Category: Fusion]]
[[Category: Maltose binding protein]]
[[Category: Mbp]]
[[Category: Nucleotide salvage pathway]]
[[Category: Pnpase]]
[[Category: Purine metabolism]]
[[Category: Ssgcid]]
[[Category: Transferase]]

Latest revision as of 16:32, 14 March 2024

Crystal structure of purine nucleoside phosphorylase from Entamoeba histolyticaCrystal structure of purine nucleoside phosphorylase from Entamoeba histolytica

Structural highlights

3tl6 is a 6 chain structure with sequence from Entamoeba histolytica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.65Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C4LXG4_ENTH1

See Also

3tl6, resolution 2.65Å

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