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==SOLUTION STRUCTURE OF THE SECOND RNA-BINDING DOMAIN (RBD2) OF HU ANTIGEN C (HUC)==
==SOLUTION STRUCTURE OF THE SECOND RNA-BINDING DOMAIN (RBD2) OF HU ANTIGEN C (HUC)==
<StructureSection load='1d9a' size='340' side='right'caption='[[1d9a]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
<StructureSection load='1d9a' size='340' side='right'caption='[[1d9a]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1d9a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D9A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D9A FirstGlance]. <br>
<table><tr><td colspan='2'>[[1d9a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D9A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D9A FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d8z|1d8z]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d9a OCA], [https://pdbe.org/1d9a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d9a RCSB], [https://www.ebi.ac.uk/pdbsum/1d9a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d9a ProSAT], [https://www.topsan.org/Proteins/RSGI/1d9a TOPSAN]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d9a OCA], [https://pdbe.org/1d9a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d9a RCSB], [https://www.ebi.ac.uk/pdbsum/1d9a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d9a ProSAT], [https://www.topsan.org/Proteins/RSGI/1d9a TOPSAN]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/ELAV3_MOUSE ELAV3_MOUSE]] Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. May also bind poly-A tracts via RRM 3. May be involved in neuronal differentiation and maintenance.<ref>PMID:9016658</ref> <ref>PMID:10734193</ref>
[https://www.uniprot.org/uniprot/ELAV3_MOUSE ELAV3_MOUSE] Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. May also bind poly-A tracts via RRM 3. May be involved in neuronal differentiation and maintenance.<ref>PMID:9016658</ref> <ref>PMID:10734193</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d9a ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d9a ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Hu antigen C (HuC) has three RNA-binding domains (RBDs). The N-terminal two, RBD1 and RBD2, are linked in tandem and bind to the AU-rich elements (AREs) in the 3'-untranslated region of particular mRNAs. The solution structures of HuC RBD1 and RBD2 were determined by NMR methods. The HuC RBD1 and RBD2 structures are quite similar to those of Sxl RBD1 and RBD2, respectively. The individual RBDs of HuC, RBD1 and RBD2 in isolation can interact rather weakly with the minimal ARE motif, AUUUA, while the didomain fragment, RBD1-RBD2, of HuC binds more tightly to a longer ARE RNA, UAUUUAUUUU. Chemical shift perturbations by the longer RNA on HuC RBD1-RBD2 were mapped on and around the two beta-sheets and on the C-terminal region of RBD1. The HuC RBD1-RBD2 residues that exhibited significant chemical shift perturbations coincide with those conserved in Sxl RBD1-RBD2. These data indicate that the RNA-binding characteristics of the HuC and Sxl didomain fragments are similar, even though the target RNAs and the biological functions of the proteins are different.
NMR studies on functional structures of the AU-rich element-binding domains of Hu antigen C.,Inoue M, Muto Y, Sakamoto H, Yokoyama S Nucleic Acids Res. 2000 Apr 15;28(8):1743-50. PMID:10734193<ref>PMID:10734193</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1d9a" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Lk3 transgenic mice]]
[[Category: Mus musculus]]
[[Category: Inoue, M]]
[[Category: Inoue M]]
[[Category: Muto, Y]]
[[Category: Muto Y]]
[[Category: Structural genomic]]
[[Category: Sakamoto H]]
[[Category: Sakamoto, H]]
[[Category: Yokoyama S]]
[[Category: Yokoyama, S]]
[[Category: Rna binding protein]]
[[Category: Rna-binding domain]]
[[Category: Rsgi]]

Revision as of 18:47, 13 March 2024

SOLUTION STRUCTURE OF THE SECOND RNA-BINDING DOMAIN (RBD2) OF HU ANTIGEN C (HUC)SOLUTION STRUCTURE OF THE SECOND RNA-BINDING DOMAIN (RBD2) OF HU ANTIGEN C (HUC)

Structural highlights

1d9a is a 1 chain structure with sequence from Mus musculus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

ELAV3_MOUSE Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. May also bind poly-A tracts via RRM 3. May be involved in neuronal differentiation and maintenance.[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Abe R, Sakashita E, Yamamoto K, Sakamoto H. Two different RNA binding activities for the AU-rich element and the poly(A) sequence of the mouse neuronal protein mHuC. Nucleic Acids Res. 1996 Dec 15;24(24):4895-901. PMID:9016658
  2. ↑ Inoue M, Muto Y, Sakamoto H, Yokoyama S. NMR studies on functional structures of the AU-rich element-binding domains of Hu antigen C. Nucleic Acids Res. 2000 Apr 15;28(8):1743-50. PMID:10734193
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