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==SH3 DOMAIN FROM HUMAN NEBULIN, NMR, 15 STRUCTURES==
==SH3 DOMAIN FROM HUMAN NEBULIN, NMR, 15 STRUCTURES==
<StructureSection load='1ark' size='340' side='right'caption='[[1ark]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''>
<StructureSection load='1ark' size='340' side='right'caption='[[1ark]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1ark]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ARK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ARK FirstGlance]. <br>
<table><tr><td colspan='2'>[[1ark]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ARK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ARK FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ark FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ark OCA], [https://pdbe.org/1ark PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ark RCSB], [https://www.ebi.ac.uk/pdbsum/1ark PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ark ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ark FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ark OCA], [https://pdbe.org/1ark PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ark RCSB], [https://www.ebi.ac.uk/pdbsum/1ark PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ark ProSAT]</span></td></tr>
</table>
</table>
== Disease ==
== Disease ==
[[https://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] Defects in NEB are the cause of nemaline myopathy type 2 (NEM2) [MIM:[https://omim.org/entry/256030 256030]]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination.<ref>PMID:10051637</ref>
[https://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN] Defects in NEB are the cause of nemaline myopathy type 2 (NEM2) [MIM:[https://omim.org/entry/256030 256030]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination.<ref>PMID:10051637</ref>  
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.  
[https://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN] This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ark ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ark ConSurf].
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<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The huge modular protein nebulin is located in the thin filament of striated muscle in vertebrates and is thought to bind and stabilize F-actin. The C-terminal part of human nebulin is anchored in the sarcomeric Z-disk and contains an SH3 domain, the first of such motifs to be identified in a myofibrillar protein. We have determined the nebulin SH3 sequence from several species and found it strikingly conserved. We have also shown that the SH3 transcripts are constitutively expressed in skeletal muscle tissues. As the first step towards a molecular understanding of nebulin's cellular role we have determined the three-dimensional structure of the human nebulin SH3 domain in solution by nuclear magnetic resonance (NMR) spectroscopy and compared it with other known SH3 structures. The nebulin SH3 domain has a well-defined structure in solution with a typical SH3 topology, consisting of a beta-sandwich of two triple-stranded, antiparallel beta-sheets arranged at right angles to each other and of a single turn of a 310-helix. An additional double-stranded antiparallel beta-sheet in the RT loop bends over the beta-sandwich. The derived structure reveals a remarkable similarity with a distinct subset of SH3 domains, especially in the structural features of the exposed hydrophobic patch that is thought to be the site of interaction with polyproline ligands. On the basis of this similarity, we have modelled the interaction with an appropriate polyproline ligand and attempted to delineate the characteristics of the physiological SH3-binding partner in the Z-disk. Our results represent the first step in reconstructing the structure of nebulin and are expected to contribute to our understanding of nebulin's functional role in myofibrillar assembly.
SH3 in muscles: solution structure of the SH3 domain from nebulin.,Politou AS, Millevoi S, Gautel M, Kolmerer B, Pastore A J Mol Biol. 1998 Feb 13;276(1):189-202. PMID:9514727<ref>PMID:9514727</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1ark" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Pastore, A]]
[[Category: Pastore A]]
[[Category: Politou, A S]]
[[Category: Politou AS]]
[[Category: Actin-binding]]
[[Category: Nebulin]]
[[Category: Sh3 domain]]
[[Category: Transferase]]
[[Category: Z-disk]]

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