6ufg: Difference between revisions

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<StructureSection load='6ufg' size='340' side='right'caption='[[6ufg]], [[Resolution|resolution]] 2.93&Aring;' scene=''>
<StructureSection load='6ufg' size='340' side='right'caption='[[6ufg]], [[Resolution|resolution]] 2.93&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6ufg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UFG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UFG FirstGlance]. <br>
<table><tr><td colspan='2'>[[6ufg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UFG FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.929&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CCC:CYTIDINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>CCC</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CCC:CYTIDINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>CCC</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ufg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ufg OCA], [http://pdbe.org/6ufg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ufg RCSB], [http://www.ebi.ac.uk/pdbsum/6ufg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ufg ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ufg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ufg OCA], [https://pdbe.org/6ufg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ufg RCSB], [https://www.ebi.ac.uk/pdbsum/6ufg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ufg ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
T-box riboregulators are a class of cis-regulatory RNAs that govern the bacterial response to amino acid starvation by binding, decoding and reading the aminoacylation status of specific transfer RNAs. Here we provide a high-resolution crystal structure of a full-length T-box from Mycobacterium tuberculosis that explains tRNA decoding and aminoacylation sensing by this riboregulator. Overall, the T-box consists of decoding and aminoacylation sensing modules bridged by a rigid pseudoknot structure formed by the mid-region domains. Stem-I and the Stem-II S-turn assemble a claw-like decoding module, while the antiterminator, Stem-III, and the adjacent linker form a tightly interwoven aminoacylation sensing module. The uncharged tRNA is selectively recognized by an unexpected set of favorable contacts from the linker region in the aminoacylation sensing module. A complex structure with a charged tRNA mimic shows that the extra moiety dislodges the linker, which is indicative of the possible chain of events that lead to alternative base-pairing and altered expression output.


Structural basis for tRNA decoding and aminoacylation sensing by T-box riboregulators.,Battaglia RA, Grigg JC, Ke A Nat Struct Mol Biol. 2019 Nov 18. pii: 10.1038/s41594-019-0327-6. doi:, 10.1038/s41594-019-0327-6. PMID:31740853<ref>PMID:31740853</ref>
==See Also==
 
*[[Riboswitch 3D structures|Riboswitch 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ufg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Battaglia, R A]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Grigg, J C]]
[[Category: Battaglia RA]]
[[Category: Ke, A]]
[[Category: Grigg JC]]
[[Category: Complex]]
[[Category: Ke A]]
[[Category: Riboregulator]]
[[Category: Riboswitch]]
[[Category: Rna]]

Latest revision as of 17:57, 13 March 2024

Co-crystal structure of M. tuberculosis ileS T-box in complex with tRNA-3'-OHCo-crystal structure of M. tuberculosis ileS T-box in complex with tRNA-3'-OH

Structural highlights

6ufg is a 2 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.929Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

6ufg, resolution 2.93Å

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