6nqy: Difference between revisions

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<StructureSection load='6nqy' size='340' side='right'caption='[[6nqy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='6nqy' size='340' side='right'caption='[[6nqy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6nqy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lepbp Lepbp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NQY FirstGlance]. <br>
<table><tr><td colspan='2'>[[6nqy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_biflexa_serovar_Patoc_strain_'Patoc_1_(Paris)' Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NQY FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LEPBI_I0267 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=456481 LEPBP])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nqy OCA], [http://pdbe.org/6nqy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nqy RCSB], [http://www.ebi.ac.uk/pdbsum/6nqy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nqy ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nqy OCA], [https://pdbe.org/6nqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nqy RCSB], [https://www.ebi.ac.uk/pdbsum/6nqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nqy ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/B0STJ8_LEPBP B0STJ8_LEPBP]
The protein FcpA is a unique component of the flagellar filament of spirochete bacteria belonging to the genus Leptospira. Although it plays an essential role in translational motility and pathogenicity, no structures of FcpA homologues are currently available in the PDB. Its three-dimensional structure will unveil the novel motility mechanisms that render pathogenic Leptospira particularly efficient at invading and disseminating within their hosts, causing leptospirosis in humans and animals. FcpA from L. interrogans was purified and crystallized, but despite laborious attempts no useful X ray diffraction data could be obtained. This challenge was solved by expressing a close orthologue from the related saprophytic species L. biflexa. Three different crystal forms were obtained: a primitive and a centred monoclinic form, as well as a hexagonal variant. All forms diffracted X-rays to suitable resolutions for crystallographic analyses, with the hexagonal type typically reaching the highest limits of 2.0 A and better. A variation of the quick-soaking procedure resulted in an iodide derivative that was instrumental for single-wavelength anomalous diffraction methods.


Crystallization of FcpA from Leptospira, a novel flagellar protein that is essential for pathogenesis.,San Martin F, Mechaly AE, Larrieux N, Wunder EA Jr, Ko AI, Picardeau M, Trajtenberg F, Buschiazzo A Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):123-129. doi:, 10.1107/S2053230X17002096. Epub 2017 Feb 21. PMID:28291747<ref>PMID:28291747</ref>
==See Also==
 
*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6nqy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Lepbp]]
[[Category: Buschiazzo A]]
[[Category: Buschiazzo, A]]
[[Category: Larrieux N]]
[[Category: Larrieux, N]]
[[Category: Mechaly A]]
[[Category: Mechaly, A]]
[[Category: Trajtenberg F]]
[[Category: Trajtenberg, F]]
[[Category: Flagellar filament]]
[[Category: Protein fibril]]

Latest revision as of 17:48, 13 March 2024

Flagellar protein FcpA from Leptospira biflexa / ab-centered monoclinic formFlagellar protein FcpA from Leptospira biflexa / ab-centered monoclinic form

Structural highlights

6nqy is a 2 chain structure with sequence from Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)'. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

B0STJ8_LEPBP

See Also

6nqy, resolution 2.50Å

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