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| <StructureSection load='6nqx' size='340' side='right'caption='[[6nqx]], [[Resolution|resolution]] 2.95Å' scene=''> | | <StructureSection load='6nqx' size='340' side='right'caption='[[6nqx]], [[Resolution|resolution]] 2.95Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6nqx]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lepbp Lepbp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NQX FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6nqx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_biflexa_serovar_Patoc_strain_'Patoc_1_(Paris)' Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NQX FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LEPBI_I0267 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=456481 LEPBP])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nqx OCA], [http://pdbe.org/6nqx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nqx RCSB], [http://www.ebi.ac.uk/pdbsum/6nqx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nqx ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nqx OCA], [https://pdbe.org/6nqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nqx RCSB], [https://www.ebi.ac.uk/pdbsum/6nqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nqx ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/B0STJ8_LEPBP B0STJ8_LEPBP] |
| The protein FcpA is a unique component of the flagellar filament of spirochete bacteria belonging to the genus Leptospira. Although it plays an essential role in translational motility and pathogenicity, no structures of FcpA homologues are currently available in the PDB. Its three-dimensional structure will unveil the novel motility mechanisms that render pathogenic Leptospira particularly efficient at invading and disseminating within their hosts, causing leptospirosis in humans and animals. FcpA from L. interrogans was purified and crystallized, but despite laborious attempts no useful X ray diffraction data could be obtained. This challenge was solved by expressing a close orthologue from the related saprophytic species L. biflexa. Three different crystal forms were obtained: a primitive and a centred monoclinic form, as well as a hexagonal variant. All forms diffracted X-rays to suitable resolutions for crystallographic analyses, with the hexagonal type typically reaching the highest limits of 2.0 A and better. A variation of the quick-soaking procedure resulted in an iodide derivative that was instrumental for single-wavelength anomalous diffraction methods.
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| Crystallization of FcpA from Leptospira, a novel flagellar protein that is essential for pathogenesis.,San Martin F, Mechaly AE, Larrieux N, Wunder EA Jr, Ko AI, Picardeau M, Trajtenberg F, Buschiazzo A Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):123-129. doi:, 10.1107/S2053230X17002096. Epub 2017 Feb 21. PMID:28291747<ref>PMID:28291747</ref>
| | ==See Also== |
| | | *[[Flagellar protein 3D structures|Flagellar protein 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
| |
| <div class="pdbe-citations 6nqx" style="background-color:#fffaf0;"></div>
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| == References == | |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Lepbp]]
| | [[Category: Buschiazzo A]] |
| [[Category: Buschiazzo, A]] | | [[Category: Larrieux N]] |
| [[Category: Larrieux, N]] | | [[Category: Mechaly A]] |
| [[Category: Mechaly, A]] | | [[Category: Trajtenberg F]] |
| [[Category: Trajtenberg, F]] | |
| [[Category: Flagellar filament]]
| |
| [[Category: Protein fibril]]
| |