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| <StructureSection load='6mp2' size='340' side='right'caption='[[6mp2]], [[Resolution|resolution]] 1.98Å' scene=''> | | <StructureSection load='6mp2' size='340' side='right'caption='[[6mp2]], [[Resolution|resolution]] 1.98Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6mp2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifld Bifld]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MP2 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6mp2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bifidobacterium_longum_DJO10A Bifidobacterium longum DJO10A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MP2 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BLD_0195 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=205913 BIFLD])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mp2 OCA], [http://pdbe.org/6mp2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mp2 RCSB], [http://www.ebi.ac.uk/pdbsum/6mp2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mp2 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mp2 OCA], [https://pdbe.org/6mp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mp2 RCSB], [https://www.ebi.ac.uk/pdbsum/6mp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mp2 ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/B3DQP5_BIFLD B3DQP5_BIFLD] |
| Bifidobacteria represent one of the first colonizers of human gut microbiota, providing to this ecosystem better health and nutrition. To maintain a mutualistic relationship, they have enzymes to degrade and use complex carbohydrates non-digestible by their hosts. To succeed in the densely populated gut environment, they evolved molecular strategies that remain poorly understood. Herein, we report a novel mechanism found in probiotic Bifidobacteria for the depolymerization of the ubiquitous 2-acetamido-2-deoxy-4-O-(beta-d-mannopyranosyl)-d-glucopyranose (Man-beta-1,4-GlcNAc), a disaccharide that composes the universal core of eukaryotic N-glycans. In contrast to Bacteroidetes, these Bifidobacteria have a specialist and strain-specific beta-mannosidase that contains three distinctive structural elements conferring high selectivity for Man-beta-1,4-GlcNAc: a lid that undergoes conformational changes upon substrate binding, a tryptophan residue swapped between the two dimeric subunits to accommodate the GlcNAc moiety, and a Rossmann fold subdomain strategically located near to the active site pocket. These key structural elements for Man-beta-1,4-GlcNAc specificity are highly conserved in Bifidobacterium species adapted to the gut of a wide range of social animals, including bee, pig, rabbit, and human. Together, our findings uncover an unprecedented molecular strategy employed by Bifidobacteria to selectively uptake carbohydrates from N-glycans in social hosts.
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| N-glycan Utilization by Bifidobacterium Gut Symbionts Involves a Specialist beta-Mannosidase.,Cordeiro RL, Pirolla RAS, Persinoti GF, Gozzo FC, de Giuseppe PO, Murakami MT J Mol Biol. 2019 Jan 11. pii: S0022-2836(19)30006-3. doi:, 10.1016/j.jmb.2018.12.017. PMID:30641082<ref>PMID:30641082</ref>
| | ==See Also== |
| | | *[[Mannosidase 3D structures|Mannosidase 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6mp2" style="background-color:#fffaf0;"></div>
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| == References == | |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Bifld]] | | [[Category: Bifidobacterium longum DJO10A]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Giuseppe, P O]] | | [[Category: Giuseppe PO]] |
| [[Category: Lorizolla-Cordeiro, R]] | | [[Category: Lorizolla-Cordeiro R]] |
| [[Category: Murakami, M T]] | | [[Category: Murakami MT]] |
| [[Category: Beta-mannosidase]]
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| [[Category: Family gh5]]
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| [[Category: Hydrolase]]
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| [[Category: Subfamily 18]]
| |