6m7z: Difference between revisions

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<StructureSection load='6m7z' size='340' side='right'caption='[[6m7z]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='6m7z' size='340' side='right'caption='[[6m7z]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6m7z]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii_rh Toxoplasma gondii rh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6M7Z FirstGlance]. <br>
<table><tr><td colspan='2'>[[6m7z]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii_RH Toxoplasma gondii RH]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M7Z FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BPK1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=383379 Toxoplasma gondii RH])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triphosphate--protein_phosphotransferase Triphosphate--protein phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.99.1 2.7.99.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m7z OCA], [https://pdbe.org/6m7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m7z RCSB], [https://www.ebi.ac.uk/pdbsum/6m7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m7z ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6m7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m7z OCA], [http://pdbe.org/6m7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m7z RCSB], [http://www.ebi.ac.uk/pdbsum/6m7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m7z ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/BPK1_TOXGO BPK1_TOXGO] Required for the growth, maintenance, and/or stability, and thus infectivity, of bradyzoite cysts.<ref>PMID:23291621</ref>  
Apicomplexan parasites replicate within a protective organelle, called the parasitophorous vacuole (PV). The Toxoplasma gondii PV is filled with a network of tubulated membranes, which are thought to facilitate trafficking of effectors and nutrients. Despite being critical to parasite virulence, there is scant mechanistic understanding of the network's functions. Here, we identify the parasite-secreted kinase WNG1 (With-No-Gly-loop) as a critical regulator of tubular membrane biogenesis. WNG1 family members adopt an atypical protein kinase fold lacking the glycine rich ATP-binding loop that is required for catalysis in canonical kinases. Unexpectedly, we find that WNG1 is an active protein kinase that localizes to the PV lumen and phosphorylates PV-resident proteins, several of which are essential for the formation of a functional intravacuolar network. Moreover, we show that WNG1-dependent phosphorylation of these proteins is required for their membrane association, and thus their ability to tubulate membranes. Consequently, WNG1 knockout parasites have an aberrant PV membrane ultrastructure. Collectively, our results describe a unique family of Toxoplasma kinases and implicate phosphorylation of secreted proteins as a mechanism of regulating PV development during parasite infection.
 
Divergent kinase regulates membrane ultrastructure of the Toxoplasma parasitophorous vacuole.,Beraki T, Hu X, Broncel M, Young JC, O'Shaughnessy WJ, Borek D, Treeck M, Reese ML Proc Natl Acad Sci U S A. 2019 Mar 8. pii: 1816161116. doi:, 10.1073/pnas.1816161116. PMID:30850550<ref>PMID:30850550</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6m7z" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Toxoplasma gondii rh]]
[[Category: Toxoplasma gondii RH]]
[[Category: Triphosphate--protein phosphotransferase]]
[[Category: Beraki T]]
[[Category: Beraki, T]]
[[Category: Borek DM]]
[[Category: Borek, D M]]
[[Category: Reese ML]]
[[Category: Reese, M L]]
[[Category: Atypical]]
[[Category: Kinase]]
[[Category: Signaling protein]]
[[Category: Transferase]]

Latest revision as of 17:41, 13 March 2024

A divergent kinase lacking the glycine-rich loop regulates membrane ultrastructure of the Toxoplasma parasitophorous vacuoleA divergent kinase lacking the glycine-rich loop regulates membrane ultrastructure of the Toxoplasma parasitophorous vacuole

Structural highlights

6m7z is a 6 chain structure with sequence from Toxoplasma gondii RH. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BPK1_TOXGO Required for the growth, maintenance, and/or stability, and thus infectivity, of bradyzoite cysts.[1]

References

  1. ↑ Buchholz KR, Bowyer PW, Boothroyd JC. Bradyzoite pseudokinase 1 is crucial for efficient oral infectivity of the Toxoplasma gondii tissue cyst. Eukaryot Cell. 2013 Mar;12(3):399-410. PMID:23291621 doi:10.1128/EC.00343-12

6m7z, resolution 2.50Å

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