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| <SX load='6e9e' size='340' side='right' viewer='molstar' caption='[[6e9e]], [[Resolution|resolution]] 3.40Å' scene=''> | | <SX load='6e9e' size='340' side='right' viewer='molstar' caption='[[6e9e]], [[Resolution|resolution]] 3.40Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6e9e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eubacterium_siraeum_dsm_15702 Eubacterium siraeum dsm 15702]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E9E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6E9E FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6e9e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Eubacterium_siraeum_DSM_15702 Eubacterium siraeum DSM 15702] and [https://en.wikipedia.org/wiki/Bacterium Bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E9E FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EUBSIR_02687 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=428128 Eubacterium siraeum DSM 15702])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6e9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e9e OCA], [http://pdbe.org/6e9e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e9e RCSB], [http://www.ebi.ac.uk/pdbsum/6e9e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e9e ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e9e OCA], [https://pdbe.org/6e9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e9e RCSB], [https://www.ebi.ac.uk/pdbsum/6e9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e9e ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/B0MS50_9FIRM B0MS50_9FIRM] |
| CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and their signature Cas13d ribonuclease employs guide RNAs to cleave matching target RNAs. To understand the molecular basis for Cas13d function and explain its compact molecular architecture, we resolved cryoelectron microscopy structures of Cas13d-guide RNA binary complex and Cas13d-guide-target RNA ternary complex to 3.4 and 3.3 A resolution, respectively. Furthermore, a 6.5 A reconstruction of apo Cas13d combined with hydrogen-deuterium exchange revealed conformational dynamics that have implications for RNA scanning. These structures, together with biochemical and cellular characterization, provide insights into its RNA-guided, RNA-targeting mechanism and delineate a blueprint for the rational design of improved transcriptome engineering technologies.
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| Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d.,Zhang C, Konermann S, Brideau NJ, Lotfy P, Wu X, Novick SJ, Strutzenberg T, Griffin PR, Hsu PD, Lyumkis D Cell. 2018 Sep 20;175(1):212-223.e17. doi: 10.1016/j.cell.2018.09.001. PMID:30241607<ref>PMID:30241607</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6e9e" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[CRISPR type VI|CRISPR type VI]] | | *[[CRISPR type VI|CRISPR type VI]] |
| == References ==
| |
| <references/>
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| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: Eubacterium siraeum dsm 15702]] | | [[Category: Bacterium]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Lyumkis, D]] | | [[Category: Lyumkis D]] |
| [[Category: Zhang, C]] | | [[Category: Zhang C]] |
| [[Category: Complex]]
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| [[Category: Crispr-ca]]
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| [[Category: Rna binding protein-rna complex]]
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| [[Category: Rnase]]
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