1qyg: Difference between revisions

Revision as of 06:51, 3 May 2008

ANTI-COCAINE ANTIBODY M82G2 COMPLEXED WITH BENZOYLECGONINE

OverviewOverview

Antibodies against cocaine and other drugs of abuse are the basis for diagnostic tests for the presence of those drugs in human serum. The 1.7A resolution crystal structure of the anti-cocaine monoclonal antibody M82G2 in complex with cocaine is presented. This structure determination was undertaken to establish the stereochemical features in the antibody binding site that confer specificity for cocaine, and as part of an ongoing project to understand the rules that govern molecular recognition. The cocaine-binding site can be characterized topologically as a narrow groove on the protein surface. The antibody utilizes water-mediated hydrogen bonding, and cation-pi and stacking (pi-pi) interactions to provide specificity. Comparison with the previously published structure of the anti-cocaine antibody GNC92H2 shows that binding of a small ligand can be achieved in diverse ways, both in terms of a binding site structure/topology and protein-ligand interactions.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Diversity in hapten recognition: structural study of an anti-cocaine antibody M82G2., Pozharski E, Moulin A, Hewagama A, Shanafelt AB, Petsko GA, Ringe D, J Mol Biol. 2005 Jun 10;349(3):570-82. Epub 2005 Apr 21. PMID:15885702 Page seeded by OCA on Sat May 3 06:51:29 2008

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OCA

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 [[Image:1qyg.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1qyg", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BCG:3-(BENZOYLOXY)-8-METHYL-8-AZABICYCLO[3.2.1]OCTANE-2-CARBOXYLIC+ACID'>BCG</scene>
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-|RELATEDENTRY=[[1q72|1Q72]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyg OCA], [http://www.ebi.ac.uk/pdbsum/1qyg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qyg RCSB]</span>
-}}
 '''ANTI-COCAINE ANTIBODY M82G2 COMPLEXED WITH BENZOYLECGONINE'''
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 ==About this Structure==
-QYG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYG OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYG OCA].
 ==Reference==
 Diversity in hapten recognition: structural study of an anti-cocaine antibody M82G2., Pozharski E, Moulin A, Hewagama A, Shanafelt AB, Petsko GA, Ringe D, J Mol Biol. 2005 Jun 10;349(3):570-82. Epub 2005 Apr 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15885702 15885702]
-[[Category: Mus musculus]]
-[[Category: Protein complex]]
 [[Category: Hewagama, A.]]
 [[Category: Petsko, G.]]
@@ Line 30: / Line 25: @@
 [[Category: Ringe, D.]]
 [[Category: Shanafelt, A.]]
-[[Category: anti-cocaine antibody]]
+[[Category: Anti-cocaine antibody]]
-[[Category: fab]]
+[[Category: Fab]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:51:29 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:20:50 2008''