6dnf: Difference between revisions

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<SX load='6dnf' size='340' side='right' viewer='molstar' caption='[[6dnf]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<SX load='6dnf' size='340' side='right' viewer='molstar' caption='[[6dnf]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6dnf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyphellophora_europaea_cbs_101466 Cyphellophora europaea cbs 101466]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DNF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6DNF FirstGlance]. <br>
<table><tr><td colspan='2'>[[6dnf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyphellophora_europaea_CBS_101466 Cyphellophora europaea CBS 101466]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DNF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGG:1-[GLYCEROLYLPHOSPHONYL]-2-[8-(2-HEXYL-CYCLOPROPYL)-OCTANAL-1-YL]-3-[HEXADECANAL-1-YL]-GLYCEROL'>DGG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HMPREF1541_00236 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1220924 Cyphellophora europaea CBS 101466])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGG:1-[GLYCEROLYLPHOSPHONYL]-2-[8-(2-HEXYL-CYCLOPROPYL)-OCTANAL-1-YL]-3-[HEXADECANAL-1-YL]-GLYCEROL'>DGG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6dnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dnf OCA], [http://pdbe.org/6dnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dnf RCSB], [http://www.ebi.ac.uk/pdbsum/6dnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dnf ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dnf OCA], [https://pdbe.org/6dnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dnf RCSB], [https://www.ebi.ac.uk/pdbsum/6dnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dnf ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/W2SDE2_9EURO W2SDE2_9EURO]
The mitochondrial calcium uniporter (MCU) is a highly selective calcium channel and a major route of calcium entry into mitochondria. How the channel catalyses ion permeation and achieves ion selectivity are not well understood, partly because MCU is thought to have a distinct architecture in comparison to other cellular channels. Here we report cryo-electron microscopy reconstructions of MCU channels from zebrafish and Cyphellophora europaea at 8.5 A and 3.2 A resolutions, respectively. In contrast to a previous report of pentameric stoichiometry for MCU, both channels are tetramers. The atomic model of C. europaea MCU shows that a conserved WDXXEP signature sequence forms the selectivity filter, in which calcium ions are arranged in single file. Coiled-coil legs connect the pore to N-terminal domains in the mitochondrial matrix. In C. europaea MCU, the N-terminal domains assemble as a dimer of dimers; in zebrafish MCU, they form an asymmetric crescent. The structures define principles that underlie ion permeation and calcium selectivity in this unusual channel.


Cryo-EM structures of fungal and metazoan mitochondrial calcium uniporters.,Baradaran R, Wang C, Siliciano AF, Long SB Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0331-8. doi:, 10.1038/s41586-018-0331-8. PMID:29995857<ref>PMID:29995857</ref>
==See Also==
 
*[[Mitochondrial calcium uniporter|Mitochondrial calcium uniporter]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6dnf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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__TOC__
</SX>
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[[Category: Cyphellophora europaea cbs 101466]]
[[Category: Cyphellophora europaea CBS 101466]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Baradaran, R]]
[[Category: Baradaran R]]
[[Category: Long, S B]]
[[Category: Long SB]]
[[Category: Wang, C]]
[[Category: Wang C]]
[[Category: Calcium]]
[[Category: Eukaryotic]]
[[Category: Ion channel]]
[[Category: Membrane protein]]
[[Category: Mitochondria]]

Latest revision as of 17:35, 13 March 2024

Cryo-EM structure of the mitochondrial calcium uniporter MCU from the fungus Cyphellophora europaeaCryo-EM structure of the mitochondrial calcium uniporter MCU from the fungus Cyphellophora europaea

6dnf, resolution 3.20Å

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