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| <SX load='6bp8' size='340' side='right' viewer='molstar' caption='[[6bp8]], [[Resolution|resolution]] 4.90Å' scene=''> | | <SX load='6bp8' size='340' side='right' viewer='molstar' caption='[[6bp8]], [[Resolution|resolution]] 4.90Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6bp8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BP8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6BP8 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6bp8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BP8 FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6bp7|6bp7]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.9Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mvp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bp8 OCA], [https://pdbe.org/6bp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bp8 RCSB], [https://www.ebi.ac.uk/pdbsum/6bp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bp8 ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6bp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bp8 OCA], [http://pdbe.org/6bp8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bp8 RCSB], [http://www.ebi.ac.uk/pdbsum/6bp8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bp8 ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/MVP_RAT MVP_RAT]] Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases (By similarity). | | [https://www.uniprot.org/uniprot/MVP_RAT MVP_RAT] Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases (By similarity). |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Prior crystal structures of the vault have provided clues of its structural variability but are non-conclusive due to crystal packing. Here, we obtained vaults by engineering at the N terminus of rat major vault protein (MVP) an HIV-1 Gag protein segment and determined their near-atomic resolution ( approximately 4.8 A) structures in a solution/non-crystalline environment. The barrel-shaped vaults in solution adopt two conformations, 1 and 2, both with D39 symmetry. From the N to C termini, each MVP monomer has three regions: body, shoulder, and cap. While conformation 1 is identical to one of the crystal structures, the shoulder in conformation 2 is translocated longitudinally up to 10 A, resulting in an outward-projected cap. Our structures clarify the structural discrepancies in the body region in the prior crystallography models. The vault's drug-delivery potential is highlighted by the internal disposition and structural flexibility of its Gag-loaded N-terminal extension at the barrel waist of the engineered vault.
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| Solution Structures of Engineered Vault Particles.,Ding K, Zhang X, Mrazek J, Kickhoefer VA, Lai M, Ng HL, Yang OO, Rome LH, Zhou ZH Structure. 2018 Mar 7. pii: S0969-2126(18)30054-6. doi:, 10.1016/j.str.2018.02.014. PMID:29551289<ref>PMID:29551289</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6bp8" style="background-color:#fffaf0;"></div>
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| ==See Also==
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| *[[Major vault protein|Major vault protein]]
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: Buffalo rat]]
| |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Ding, K]] | | [[Category: Rattus norvegicus]] |
| [[Category: Kickhoefer, V A]] | | [[Category: Ding K]] |
| [[Category: Lai, M]] | | [[Category: Kickhoefer VA]] |
| [[Category: Mrazek, J]] | | [[Category: Lai M]] |
| [[Category: Ng, H L]] | | [[Category: Mrazek J]] |
| [[Category: Rome, L H]] | | [[Category: Ng HL]] |
| [[Category: Yang, O O]] | | [[Category: Rome LH]] |
| [[Category: Zhang, X]] | | [[Category: Yang OO]] |
| [[Category: Zhou, Z H]] | | [[Category: Zhang X]] |
| [[Category: Structural protein]]
| | [[Category: Zhou ZH]] |
| [[Category: Vault recombinant protein structure engineered nano-particle]]
| |