|
|
| Line 3: |
Line 3: |
| <StructureSection load='6bar' size='340' side='right'caption='[[6bar]], [[Resolution|resolution]] 2.91Å' scene=''> | | <StructureSection load='6bar' size='340' side='right'caption='[[6bar]], [[Resolution|resolution]] 2.91Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6bar]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BAR FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6bar]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BAR FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.908Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTHA1241 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bar OCA], [http://pdbe.org/6bar PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bar RCSB], [http://www.ebi.ac.uk/pdbsum/6bar PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bar ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bar OCA], [https://pdbe.org/6bar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bar RCSB], [https://www.ebi.ac.uk/pdbsum/6bar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bar ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/Q5SIX3_THET8 Q5SIX3_THET8]] Peptidoglycan polymerase that is essential for cell wall elongation.[HAMAP-Rule:MF_02079] | | [https://www.uniprot.org/uniprot/Q5SIX3_THET8 Q5SIX3_THET8] Peptidoglycan polymerase that is essential for cell wall elongation.[HAMAP-Rule:MF_02079] |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| The shape, elongation, division and sporulation (SEDS) proteins are a large family of ubiquitous and essential transmembrane enzymes with critical roles in bacterial cell wall biology. The exact function of SEDS proteins was for a long time poorly understood, but recent work has revealed that the prototypical SEDS family member RodA is a peptidoglycan polymerase-a role previously attributed exclusively to members of the penicillin-binding protein family. This discovery has made RodA and other SEDS proteins promising targets for the development of next-generation antibiotics. However, little is known regarding the molecular basis of SEDS activity, and no structural data are available for RodA or any homologue thereof. Here we report the crystal structure of Thermus thermophilus RodA at a resolution of 2.9 A, determined using evolutionary covariance-based fold prediction to enable molecular replacement. The structure reveals a ten-pass transmembrane fold with large extracellular loops, one of which is partially disordered. The protein contains a highly conserved cavity in the transmembrane domain, reminiscent of ligand-binding sites in transmembrane receptors. Mutagenesis experiments in Bacillus subtilis and Escherichia coli show that perturbation of this cavity abolishes RodA function both in vitro and in vivo, indicating that this cavity is catalytically essential. These results provide a framework for understanding bacterial cell wall synthesis and SEDS protein function.
| |
| | |
| Structure of the peptidoglycan polymerase RodA resolved by evolutionary coupling analysis.,Sjodt M, Brock K, Dobihal G, Rohs PDA, Green AG, Hopf TA, Meeske AJ, Srisuknimit V, Kahne D, Walker S, Marks DS, Bernhardt TG, Rudner DZ, Kruse AC Nature. 2018 Apr 5;556(7699):118-121. doi: 10.1038/nature25985. Epub 2018 Mar 28. PMID:29590088<ref>PMID:29590088</ref>
| |
| | |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 6bar" style="background-color:#fffaf0;"></div>
| |
|
| |
|
| ==See Also== | | ==See Also== |
| *[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]] | | *[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]] |
| == References ==
| |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Thet8]] | | [[Category: Thermus thermophilus HB8]] |
| [[Category: Bernhardt, T G]] | | [[Category: Bernhardt TG]] |
| [[Category: Brock, K]] | | [[Category: Brock K]] |
| [[Category: Dobihal, G]] | | [[Category: Dobihal G]] |
| [[Category: Green, A G]] | | [[Category: Green AG]] |
| [[Category: Hopf, T A]] | | [[Category: Hopf TA]] |
| [[Category: Kruse, A C]] | | [[Category: Kruse AC]] |
| [[Category: Marks, D S]] | | [[Category: Marks DS]] |
| [[Category: Meeske, A J]] | | [[Category: Meeske AJ]] |
| [[Category: Rohs, P D.A]] | | [[Category: Rohs PDA]] |
| [[Category: Rudner, D Z]] | | [[Category: Rudner DZ]] |
| [[Category: Sjodt, M]] | | [[Category: Sjodt M]] |
| [[Category: Elongasome]]
| |
| [[Category: Peptidoglycan glycosyltransferase]]
| |
| [[Category: Shape elogation division and sporulation]]
| |
| [[Category: Transferase]]
| |
| [[Category: Transmembrane protein]]
| |