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| == Function == | | == Function == |
| [https://www.uniprot.org/uniprot/Q0S6D0_RHOJR Q0S6D0_RHOJR] | | [https://www.uniprot.org/uniprot/Q0S6D0_RHOJR Q0S6D0_RHOJR] |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| CouR, a MarR-type transcriptional repressor, regulates the cou genes, encoding p-hydroxycinnamate catabolism in the soil bacterium Rhodococcus jostii RHA1. The CouR dimer bound two molecules of the catabolite p-coumaroyl-CoA (Kd = 11 +/- 1 muM). The presence of p-coumaroyl-CoA, but neither p-coumarate nor CoASH, abrogated CouR's binding to its operator DNA in vitro. The crystal structures of ligand-free CouR and its p-coumaroyl-CoA-bound form showed no significant conformational differences, in contrast to other MarR regulators. The CouR-p-coumaroyl-CoA structure revealed two ligand molecules bound to the CouR dimer with their phenolic moieties occupying equivalent hydrophobic pockets in each protomer and their CoA moieties adopting non-equivalent positions to mask the regulator's predicted DNA-binding surface. More specifically, the CoA phosphates formed salt bridges with predicted DNA-binding residues Arg36 and Arg38, changing the overall charge of the DNA-binding surface. The substitution of either arginine with alanine completely abrogated the ability of CouR to bind DNA. By contrast, the R36A/R38A double variant retained a relatively high affinity for p-coumaroyl-CoA (Kd = 89 +/- 6 muM). Together, our data point to a novel mechanism of action in which the ligand abrogates the repressor's ability to bind DNA by steric occlusion of key DNA-binding residues and charge repulsion of the DNA backbone.
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| The activity of CouR, a MarR family transcriptional regulator, is modulated through a novel molecular mechanism.,Otani H, Stogios PJ, Xu X, Nocek B, Li SN, Savchenko A, Eltis LD Nucleic Acids Res. 2016 Jan 29;44(2):595-607. doi: 10.1093/nar/gkv955. Epub 2015 , Sep 22. PMID:26400178<ref>PMID:26400178</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 5cyv" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] | | *[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |