2pk0: Difference between revisions

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<StructureSection load='2pk0' size='340' side='right'caption='[[2pk0]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
<StructureSection load='2pk0' size='340' side='right'caption='[[2pk0]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2pk0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Stra1 Stra1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PK0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2pk0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_A909 Streptococcus agalactiae A909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PK0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">stp ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=205921 STRA1])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pk0 OCA], [https://pdbe.org/2pk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pk0 RCSB], [https://www.ebi.ac.uk/pdbsum/2pk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pk0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pk0 OCA], [https://pdbe.org/2pk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pk0 RCSB], [https://www.ebi.ac.uk/pdbsum/2pk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pk0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8VQA1_STRAG Q8VQA1_STRAG]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pk0 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pk0 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We solved the crystal structure of Streptococcus agalactiae serine/threonine phosphatase (SaSTP) using a combination of single-wavelength anomalous dispersion phasing and molecular replacement. The overall structure resembles that of previously characterized members of the PPM/PP2C STP family. The asymmetric unit contains four monomers and we observed two novel conformations for the flap domain among them. In one of these conformations, the enzyme binds three metal ions, whereas in the other it binds only two. The three-metal ion structure also has the active site arginine in a novel conformation. The switch between the two- and three-metal ion structures appears to be binding of another monomer to the active site of STP, which promotes binding of the third metal ion. This interaction may mimic the binding of a product complex, especially since the motif binding to the active site contains a serine residue aligning remarkably well with the phosphate found in the human STP structure.
Structure of Streptococcus agalactiae serine/threonine phosphatase. The subdomain conformation is coupled to the binding of a third metal ion.,Rantanen MK, Lehtio L, Rajagopal L, Rubens CE, Goldman A FEBS J. 2007 Jun;274(12):3128-37. Epub 2007 May 22. PMID:17521332<ref>PMID:17521332</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2pk0" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]]
*[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Phosphoprotein phosphatase]]
[[Category: Streptococcus agalactiae A909]]
[[Category: Stra1]]
[[Category: Goldman A]]
[[Category: Goldman, A]]
[[Category: Lehtio L]]
[[Category: Lehtio, L]]
[[Category: Rajagopal L]]
[[Category: Rajagopal, L]]
[[Category: Rantanen MK]]
[[Category: Rantanen, M K]]
[[Category: Rubens CE]]
[[Category: Rubens, C E]]
[[Category: Phosphatase]]
[[Category: Serine]]
[[Category: Signaling motif]]
[[Category: Signaling protein]]
[[Category: Streptococcus agalactiae]]
[[Category: Threonine]]

Latest revision as of 16:53, 13 March 2024

Structure of the S. agalactiae serine/threonine phosphatase at 2.65 resolutionStructure of the S. agalactiae serine/threonine phosphatase at 2.65 resolution

Structural highlights

2pk0 is a 4 chain structure with sequence from Streptococcus agalactiae A909. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.65Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8VQA1_STRAG

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2pk0, resolution 2.65Å

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OCA
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