1qx1: Difference between revisions

Revision as of 06:48, 3 May 2008

Golgi alpha-mannosidase II D341N mutant complex with 2-F-mannosyl-F

OverviewOverview

The family 38 golgi alpha-mannosidase II, thought to cleave mannosidic bonds through a double displacement mechanism involving a reaction intermediate, is a clinically important enzyme involved in glycoprotein processing. The structure of three different covalent glycosyl-enzyme intermediates have been determined to 1.2-A resolution for the Golgi alpha-mannosidase II from Drosophila melanogaster by use of fluorinated sugar analogues, both with the wild-type enzyme and a mutant enzyme in which the acid/base catalyst has been removed. All these structures reveal sugar intermediates bound in a distorted 1S5 skew boat conformation. The similarity of this conformation with that of the substrate in the recently determined structure of the Michaelis complex of a beta-mannanase (Ducros, V. M. A., Zechel, D. L., Murshudov, G. N., Gilbert, H. J., Szabo, L., Stoll, D., Withers, S. G., and Davies, G. J. (2002) Angew. Chem. Int. Ed. Engl. 41, 2824-2827) suggests that these disparate enzymes have recruited common stereoelectronic features in evolving their catalytic mechanisms.

About this StructureAbout this Structure

1QX1 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

ReferenceReference

Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates., Numao S, Kuntz DA, Withers SG, Rose DR, J Biol Chem. 2003 Nov 28;278(48):48074-83. Epub 2003 Sep 5. PMID:12960159 Page seeded by OCA on Sat May 3 06:48:13 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1qx1.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1qx1 |SIZE=350|CAPTION= <scene name='initialview01'>1qx1</scene>, resolution 1.30&Aring;
+The line below this paragraph, containing "STRUCTURE_1qx1", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FMF:2-DEOXY-2-FLUOROHEXOPYRANOSYL+FLUORIDE'>FMF</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannosyl-oligosaccharide_1,3-1,6-alpha-mannosidase Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.114 3.2.1.114] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= ALPHA-MAN-II OR GMII OR CG18474/CG18802/CG8139 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])
+-->
-|DOMAIN=
+{{STRUCTURE_1qx1|  PDB=1qx1  |  SCENE=  }}
-|RELATEDENTRY=[[1qwn|1QWN]], [[1qwu|1QWU]], [[1ps3|1PS3]], [[1hty|1HTY]], [[1hww|1HWW]], [[1hxk|1HXK]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qx1 OCA], [http://www.ebi.ac.uk/pdbsum/1qx1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qx1 RCSB]</span>
-}}
 '''Golgi alpha-mannosidase II D341N mutant complex with 2-F-mannosyl-F'''
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 [[Category: Rose, D R.]]
 [[Category: Withers, S G.]]
-[[Category: covalent catalytic intermediate]]
+[[Category: Covalent catalytic intermediate]]
-[[Category: glycosyl hydrolase family 38]]
+[[Category: Glycosyl hydrolase family 38]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:48:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:20:17 2008''