1qwz: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1qwz.jpg|left|200px]] | [[Image:1qwz.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1qwz", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_1qwz| PDB=1qwz | SCENE= }} | |||
}} | |||
'''Crystal structure of Sortase B from S. aureus complexed with MTSET''' | '''Crystal structure of Sortase B from S. aureus complexed with MTSET''' | ||
| Line 29: | Line 26: | ||
[[Category: Schneewind, O.]] | [[Category: Schneewind, O.]] | ||
[[Category: Zong, Y.]] | [[Category: Zong, Y.]] | ||
[[Category: | [[Category: Beta barrel]] | ||
[[Category: | [[Category: Transpeptidase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:47:57 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 06:47, 3 May 2008
Crystal structure of Sortase B from S. aureus complexed with MTSET
OverviewOverview
Many surface proteins of Gram-positive bacteria, which play important roles during the pathogenesis of human infections, are anchored to the cell wall envelope by a mechanism requiring sortases. Sortase B, a cysteine transpeptidase from Staphylococcus aureus, cleaves the C-terminal sorting signal of IsdC at the NPQTN motif and tethers the polypeptide to the pentaglycine cell wall cross-bridge. During catalysis, the active site cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges. We report here the crystal structures of SrtBDeltaN30 in complex with two active site inhibitors, MTSET and E64, and with the cell wall substrate analog tripleglycine. These structures reveal, for the first time, the active site disposition and the unique Cys-Arg catalytic machinery of the cysteine transpeptidase, and they also provide useful information for the future design of anti-infective agents against sortases.
About this StructureAbout this Structure
1QWZ is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
ReferenceReference
The structure of sortase B, a cysteine transpeptidase that tethers surface protein to the Staphylococcus aureus cell wall., Zong Y, Mazmanian SK, Schneewind O, Narayana SV, Structure. 2004 Jan;12(1):105-12. PMID:14725770 Page seeded by OCA on Sat May 3 06:47:57 2008