2aus: Difference between revisions

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<StructureSection load='2aus' size='340' side='right'caption='[[2aus]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='2aus' size='340' side='right'caption='[[2aus]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2aus]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"pyrococcus_abyssi"_erauso_et_al._1993 "pyrococcus abyssi" erauso et al. 1993]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AUS FirstGlance]. <br>
<table><tr><td colspan='2'>[[2aus]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AUS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aus OCA], [https://pdbe.org/2aus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aus RCSB], [https://www.ebi.ac.uk/pdbsum/2aus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aus ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aus OCA], [https://pdbe.org/2aus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aus RCSB], [https://www.ebi.ac.uk/pdbsum/2aus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aus ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/TRUB_PYRAB TRUB_PYRAB]] Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs (By similarity). [[https://www.uniprot.org/uniprot/NOP10_PYRAB NOP10_PYRAB]] Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA (By similarity).  
[https://www.uniprot.org/uniprot/TRUB_PYRAB TRUB_PYRAB] Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aus ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aus ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In archaeal rRNAs, the isomerization of uridine into pseudouridine (Psi) is achieved by the H/ACA sRNPs and the minimal set of proteins required for RNA:Psi-synthase activity is the aCBF5-aNOP10 protein pair. The crystal structure of the aCBF5-aNOP10 heterodimer from Pyrococcus abyssi was solved at 2.1 A resolution. In this structure, protein aNOP10 has an extended shape, with a zinc-binding motif at the N-terminus and an alpha-helix at the C-terminus. Both motifs contact the aCBF5 catalytic domain. Although less efficiently as does the full-length aNOP10, the aNOP10 C-terminal domain binds aCBF5 and stimulates the RNA-guided activity. We show that the C-terminal domain of aCBF5 (the PUA domain), which is wrapped by an N-terminal extension of aCBF5, plays a crucial role for aCBF5 binding to the guide sRNA. Addition of this domain in trans partially complement particles assembled with an aCBF5DeltaPUA truncated protein. In the crystal structure, the aCBF5-aNOP10 complex forms two kinds of heterotetramers with parallel and perpendicular orientations of the aNOP10 terminal alpha-helices, respectively. By gel filtration assay, we showed that aNOP10 can dimerize in solution. As both residues Y41 and L48 were needed for dimerization, the dimerization likely takes place by interaction of parallel alpha-helices.
Crystal structure determination and site-directed mutagenesis of the Pyrococcus abyssi aCBF5-aNOP10 complex reveal crucial roles of the C-terminal domains of both proteins in H/ACA sRNP activity.,Manival X, Charron C, Fourmann JB, Godard F, Charpentier B, Branlant C Nucleic Acids Res. 2006 Feb 2;34(3):826-39. Print 2006. PMID:16456033<ref>PMID:16456033</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2aus" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
*[[Ribosome biogenesis protein|Ribosome biogenesis protein]]
*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Pyrococcus abyssi erauso et al. 1993]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Branlant, C]]
[[Category: Pyrococcus abyssi]]
[[Category: Charpentier, B]]
[[Category: Branlant C]]
[[Category: Charron, C]]
[[Category: Charpentier B]]
[[Category: Fourmann, J B]]
[[Category: Charron C]]
[[Category: Godard, F]]
[[Category: Fourmann J-B]]
[[Category: Manival, X]]
[[Category: Godard F]]
[[Category: Isomerase]]
[[Category: Manival X]]
[[Category: Isomerase-structural protein complex]]
[[Category: Structural protein]]

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