5tcl: Difference between revisions

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<StructureSection load='5tcl' size='340' side='right'caption='[[5tcl]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<StructureSection load='5tcl' size='340' side='right'caption='[[5tcl]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5tcl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TCL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5TCL FirstGlance]. <br>
<table><tr><td colspan='2'>[[5tcl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TCL FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.201&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5tcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tcl OCA], [http://pdbe.org/5tcl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tcl RCSB], [http://www.ebi.ac.uk/pdbsum/5tcl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tcl ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tcl OCA], [https://pdbe.org/5tcl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tcl RCSB], [https://www.ebi.ac.uk/pdbsum/5tcl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tcl ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/THM1_THADA THM1_THADA]] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.  
[https://www.uniprot.org/uniprot/THM1_THADA THM1_THADA] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phasing of novel macromolecular crystal structures has been challenging since the start of structural biology. Making use of anomalous diffraction of natively present elements, such as sulfur and phosphorus, for phasing has been possible for some systems, but hindered by the necessity to access longer X-ray wavelengths in order to make most use of the anomalous scattering contributions of these elements. Presented here are the results from a first successful experimental phasing study of a macromolecular crystal structure at a wavelength close to the sulfur K edge. This has been made possible by the in-vacuum setup and the long-wavelength optimised experimental setup at the I23 beamline at Diamond Light Source. In these early commissioning experiments only standard data collection and processing procedures have been applied, in particular no dedicated absorption correction has been used. Nevertheless the success of the experiment demonstrates that the capability to extract phase information can be even further improved once data collection protocols and data processing have been optimised.
 
Long-wavelength macromolecular crystallography - First successful native SAD experiment close to the sulfur edge.,Aurelius O, Duman R, El Omari K, Mykhaylyk V, Wagner A Nucl Instrum Methods Phys Res B. 2017 Nov 15;411:12-16. doi:, 10.1016/j.nimb.2016.12.005. PMID:29276323<ref>PMID:29276323</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5tcl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Thaumatococcus daniellii]]
[[Category: Thaumatococcus daniellii]]
[[Category: Aurelius, O]]
[[Category: Aurelius O]]
[[Category: Duman, R]]
[[Category: Duman R]]
[[Category: Mykhaylyk, V]]
[[Category: El Omari K]]
[[Category: Omari, K El]]
[[Category: Mykhaylyk V]]
[[Category: Wagner, A]]
[[Category: Wagner A]]
[[Category: Long wavelength]]
[[Category: Plant protein]]
[[Category: S-sad]]
[[Category: Vacuum]]

Latest revision as of 18:39, 6 March 2024

Thaumatin from 4.96 A wavelength data collectionThaumatin from 4.96 A wavelength data collection

Structural highlights

5tcl is a 1 chain structure with sequence from Thaumatococcus daniellii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.201Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

THM1_THADA Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.

5tcl, resolution 3.20Å

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OCA
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