5uqq: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
Line 3: Line 3:
<StructureSection load='5uqq' size='340' side='right'caption='[[5uqq]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='5uqq' size='340' side='right'caption='[[5uqq]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5uqq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfm Aspfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UQQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5UQQ FirstGlance]. <br>
<table><tr><td colspan='2'>[[5uqq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UQQ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5uqo|5uqo]], [[5uqs|5uqs]], [[5uqr|5uqr]], [[5uqu|5uqu]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mcsA, cit1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 ASPFM])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uqq OCA], [https://pdbe.org/5uqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uqq RCSB], [https://www.ebi.ac.uk/pdbsum/5uqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uqq ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5uqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uqq OCA], [http://pdbe.org/5uqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uqq RCSB], [http://www.ebi.ac.uk/pdbsum/5uqq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uqq ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/PRPC_ASPFM PRPC_ASPFM]] Component of the methylcitrate cycle that catalyzes the synthesis of (2S,3S)-2-methylcitrate from propionyl-CoA and oxaloacetate. Plays an important role in detoxification of propionyl-CoA, an inhibitor of both primary and secondary metabolism. Has also citrate synthase activity using as substrates acetyl-CoA and oxaloacetate. May play a role in virulence.<ref>PMID:16008561</ref> 
[https://www.uniprot.org/uniprot/PRPC_ASPFM PRPC_ASPFM] Component of the methylcitrate cycle that catalyzes the synthesis of (2S,3S)-2-methylcitrate from propionyl-CoA and oxaloacetate. Plays an important role in detoxification of propionyl-CoA, an inhibitor of both primary and secondary metabolism. Has also citrate synthase activity using as substrates acetyl-CoA and oxaloacetate. May play a role in virulence.<ref>PMID:16008561</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Aspergillus fumigatus is a ubiquitous fungus that is not only a problem in agriculture, but also in healthcare. Aspergillus fumigatus drug resistance is becoming more prominent which is mainly attributed to the widespread use of fungicides in agriculture. The fungi-specific 2-methylcitrate cycle is responsible for detoxifying propionyl-CoA, a toxic metabolite produced as the fungus breaks down proteins and amino acids. The enzyme responsible for this detoxification is 2-methylcitrate synthase (mcsA) and is a potential candidate for the design of new anti-fungals. However, mcsA is very similar in structure to human citrate synthase (hCS) and catalyzes the same reaction. Therefore, both enzymes were studied in parallel to provide foundations for design of mcsA-specific inhibitors. The first crystal structures of citrate synthase from humans and 2-methylcitrate synthase from A. fumigatus are reported. The determined structures capture various conformational states of the enzymes and several inhibitors were identified and characterized. Despite a significant homology, mcsA and hCS display pronounced differences in substrate specificity and cooperativity. Considering that the active sites of the enzymes are almost identical, the differences in reactions catalyzed by enzymes are caused by residues that are in the vicinity of the active site and influence conformational changes of the enzymes.
 
Comparative studies of Aspergillus fumigatus 2-methylcitrate synthase and human citrate synthase.,Schlachter CR, Klapper V, Radford T, Chruszcz M Biol Chem. 2019 Nov 26;400(12):1567-1581. doi: 10.1515/hsz-2019-0106. PMID:31141475<ref>PMID:31141475</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5uqq" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
Line 28: Line 18:
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aspfm]]
[[Category: Aspergillus fumigatus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chruszcz, M]]
[[Category: Chruszcz M]]
[[Category: Schlachter, C]]
[[Category: Schlachter C]]
[[Category: 2-methylcitrate synthase]]
[[Category: Citrate synthase]]
[[Category: Mcsa]]
[[Category: Transferase]]

Latest revision as of 17:28, 6 March 2024

Crystal structure of 2-methylcitrate synthase from Aspergillus fumigatusCrystal structure of 2-methylcitrate synthase from Aspergillus fumigatus

Structural highlights

5uqq is a 6 chain structure with sequence from Aspergillus fumigatus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PRPC_ASPFM Component of the methylcitrate cycle that catalyzes the synthesis of (2S,3S)-2-methylcitrate from propionyl-CoA and oxaloacetate. Plays an important role in detoxification of propionyl-CoA, an inhibitor of both primary and secondary metabolism. Has also citrate synthase activity using as substrates acetyl-CoA and oxaloacetate. May play a role in virulence.[1]

See Also

References

  1. ↑ Maerker C, Rohde M, Brakhage AA, Brock M. Methylcitrate synthase from Aspergillus fumigatus. Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia. FEBS J. 2005 Jul;272(14):3615-30. doi: 10.1111/j.1742-4658.2005.04784.x. PMID:16008561 doi:http://dx.doi.org/10.1111/j.1742-4658.2005.04784.x

5uqq, resolution 2.30Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=5uqq&oldid=4082808"