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| <StructureSection load='5txh' size='340' side='right'caption='[[5txh]], [[Resolution|resolution]] 1.45Å' scene=''> | | <StructureSection load='5txh' size='340' side='right'caption='[[5txh]], [[Resolution|resolution]] 1.45Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[5txh]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TXH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5TXH FirstGlance]. <br> | | <table><tr><td colspan='2'>[[5txh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TXH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TXH FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5txj|5txj]], [[5txd|5txd]]</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5txh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5txh OCA], [http://pdbe.org/5txh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5txh RCSB], [http://www.ebi.ac.uk/pdbsum/5txh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5txh ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5txh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5txh OCA], [https://pdbe.org/5txh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5txh RCSB], [https://www.ebi.ac.uk/pdbsum/5txh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5txh ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Amyloid formation is associated with devastating diseases such as Alzheimer's, Parkinson's and Type-2 diabetes. The large amyloid deposits found in patients suffering from these diseases have remained difficult to probe by structural means. Recent NMR models also predict heterotypic interactions from distinct peptide fragments but limited evidence of heterotypic packed sheets is observed in solution. Here we characterize two segments of the protein amyloid beta (Abeta) known to form fibrils in Alzheimer's disease patients. We designed two variants of Abeta(19-24) and Abeta(27-32), IFAEDV (I6V) and NKGAIF (N6F) to lower the aggregation propensity of individual peptides while maintaining the similar interactions between the two segments in their native forms. We found that the variants do not form significant amyloid fibrils individually but a 1:1 mixture forms abundant fibrils. Using ion mobility-mass spectrometry (IM-MS), hetero-oligomers up to decamers were found in the mixture while the individual peptides formed primarily dimers and some tetramers consistent with a strong heterotypic interaction between the two segments. We showed by X-ray crystallography that I6V formed a Class 7 zipper with a weakly packed pair of beta-sheets and no segregated dry interface, while N6F formed a more stable Class 1 zipper. In a mixture of equimolar N6F:I6V, I6V forms a more stable zipper than in I6V alone while no N6F or hetero-typic zippers are observed. These data are consistent with a mechanism where N6F catalyzes assembly of I6V into a stable zipper and perhaps into stable, pure I6V fibrils that are observed in AFM measurements.
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| Distal amyloid beta-protein fragments template amyloid assembly.,Do TD, Sangwan S, de Almeida NEC, Ilitchev AI, Giammona M, Sawaya MR, Buratto SK, Eisenberg DS, Bowers MT Protein Sci. 2018 Jul;27(7):1181-1190. doi: 10.1002/pro.3375. Epub 2018 Feb 16. PMID:29349888<ref>PMID:29349888</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 5txh" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| | [[Category: Homo sapiens]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Eisenberg, D]] | | [[Category: Eisenberg D]] |
| [[Category: Sangwan, S]] | | [[Category: Sangwan S]] |
| [[Category: Sawaya, M R]] | | [[Category: Sawaya MR]] |
| [[Category: De novo protein]]
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| [[Category: Protein fibril]]
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