1qrk: Difference between revisions
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'''HUMAN FACTOR XIII WITH STRONTIUM BOUND IN THE ION SITE''' | '''HUMAN FACTOR XIII WITH STRONTIUM BOUND IN THE ION SITE''' | ||
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==About this Structure== | ==About this Structure== | ||
1QRK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry | 1QRK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bl2 1bl2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QRK OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Trong, I Le.]] | [[Category: Trong, I Le.]] | ||
[[Category: Yee, V C.]] | [[Category: Yee, V C.]] | ||
[[Category: | [[Category: Blood coagulation]] | ||
[[Category: | [[Category: Calcium]] | ||
[[Category: | [[Category: Strontium]] | ||
[[Category: | [[Category: Transglutaminase]] | ||
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Revision as of 06:37, 3 May 2008
HUMAN FACTOR XIII WITH STRONTIUM BOUND IN THE ION SITE
OverviewOverview
The presence or absence of calcium determines the activation, activity, oligomerization, and stability of blood coagulation factor XIII. To explore these observed effects, we have determined the x-ray crystal structure of recombinant factor XIII A2 in the presence of calcium, strontium, and ytterbium. The main calcium binding site within each monomer involves the main chain oxygen atom of Ala-457, and also the side chains from residues Asn-436, Asp-438, Glu-485, and Glu-490. Calcium and strontium bind in the same location, while ytterbium binds several angstroms removed. A novel ytterbium binding site is also found at the dimer two-fold axis, near residues Asp-270 and Glu-272, and this site may be related to the reported inhibition by lanthanide metals (Achyuthan, K. E., Mary, A., and Greenberg, C. S. (1989) Biochem. J. 257, 331-338). The overall structure of ion-bound factor XIII is very similar to the previously determined crystal structures of factor XIII zymogen, likely due to the constraints of this monoclinic crystal form. We have merged the three independent sets of water molecules in the structures to determine which water molecules are conserved and possibly structurally significant.
About this StructureAbout this Structure
1QRK is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 1bl2. Full crystallographic information is available from OCA.
ReferenceReference
Identification of the calcium binding site and a novel ytterbium site in blood coagulation factor XIII by x-ray crystallography., Fox BA, Yee VC, Pedersen LC, Le Trong I, Bishop PD, Stenkamp RE, Teller DC, J Biol Chem. 1999 Feb 19;274(8):4917-23. PMID:9988734 Page seeded by OCA on Sat May 3 06:37:21 2008