1qpp: Difference between revisions

Revision as of 06:33, 3 May 2008

CRYSTAL STRUCTURES OF SELF CAPPING PAPD CHAPERONE HOMODIMERS

OverviewOverview

PapD is an immunoglobulin-like chaperone that mediates the assembly of P pili in uropathogenic strains of Escherichia coli. It binds and caps interactive surfaces on pilus subunits to prevent their premature associations in the periplasm. We elucidated the structural basis of a mechanism whereby PapD also interacts with itself, capping its own subunit binding surface. Crystal structures of dimeric forms of PapD revealed that this self-capping mechanism involves a rearrangement and ordering of the C2-D2 and F1-G1 loops upon dimerization which might ensure that a stable dimer is not formed in solution in spite of a relatively large dimer interface. An analysis of site directed mutations revealed that chaperone dimerization requires the same surface that is otherwise used to bind subunits.

About this StructureAbout this Structure

1QPP is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of chaperone self-capping in P pilus biogenesis., Hung DL, Pinkner JS, Knight SD, Hultgren SJ, Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):8178-83. PMID:10393968 Page seeded by OCA on Sat May 3 06:33:37 2008

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OCA

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 [[Image:1qpp.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpp OCA], [http://www.ebi.ac.uk/pdbsum/1qpp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qpp RCSB]</span>
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 '''CRYSTAL STRUCTURES OF SELF CAPPING PAPD CHAPERONE HOMODIMERS'''
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 [[Category: Knight, S D.]]
 [[Category: Pinkner, J S.]]
-[[Category: beta barrel]]
+[[Category: Beta barrel]]
-[[Category: immunoglobulin fold chaperone]]
+[[Category: Immunoglobulin fold chaperone]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:33:37 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:17:15 2008''