1qnq: Difference between revisions

Revision as of 06:29, 3 May 2008

THE 3-D STRUCTURE OF A TRICHODERMA REESEI B-MANNANASE FROM GLYCOSIDE HYDROLASE FAMILY 5

OverviewOverview

The crystal structure of the catalytic core domain of beta-mannanase from the fungus Trichoderma reesei has been determined at a resolution of 1.5 A. The structure was solved using the anomalous scattering from a single non-isomorphous platinum complex with two heavy-metal sites in space group P2(1). The map computed with the experimental phases was enhanced by the application of an automated model building and refinement procedure using the amplitudes and experimental phases as observations. This approach is expected to be of more general application. The structure of the native enzyme and complexes with Tris-HCl and mannobiose are also reported: the mannobiose binds in subsites +1 and +2. The structure is briefly compared with that of the homologous beta-mannanase from the bacterium Thermomonospora fusca.

About this StructureAbout this Structure

1QNQ is a Single protein structure of sequence from Hypocrea jecorina. Full crystallographic information is available from OCA.

ReferenceReference

The three-dimensional structure of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5., Sabini E, Schubert H, Murshudov G, Wilson KS, Siika-Aho M, Penttila M, Acta Crystallogr D Biol Crystallogr. 2000 Jan;56(Pt 1):3-13. PMID:10666621 Page seeded by OCA on Sat May 3 06:29:38 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1qnq.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1qnq |SIZE=350|CAPTION= <scene name='initialview01'>1qnq</scene>, resolution 1.65&Aring;
+The line below this paragraph, containing "STRUCTURE_1qnq", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=MAN:Mannan+Binding+Site'>MAN</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TPT:2,2&#39;&#58;6&#39;,2&#39;&#39;-TERPYRIDINE+PLATINUM(II)'>TPT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1qnq|  PDB=1qnq  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qnq OCA], [http://www.ebi.ac.uk/pdbsum/1qnq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qnq RCSB]</span>
-}}
 '''THE 3-D STRUCTURE OF A TRICHODERMA REESEI B-MANNANASE FROM GLYCOSIDE HYDROLASE FAMILY 5'''
@@ Line 32: / Line 29: @@
 [[Category: Siika-Aho, M.]]
 [[Category: Wilson, K S.]]
-[[Category: anomalous scattering]]
+[[Category: Anomalous scattering]]
-[[Category: mannanase]]
+[[Category: Mannanase]]
-[[Category: trichoderma reesei]]
+[[Category: Trichoderma reesei]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:29:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:16:23 2008''