3txm: Difference between revisions

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<StructureSection load='3txm' size='340' side='right'caption='[[3txm]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
<StructureSection load='3txm' size='340' side='right'caption='[[3txm]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3txm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TXM FirstGlance]. <br>
<table><tr><td colspan='2'>[[3txm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TXM FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GD3:GADOLINIUM+ION'>GD3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3txn|3txn]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GD3:GADOLINIUM+ION'>GD3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BcDNA.LD18931, CG10149, Dmel_CG10149, Rpn6 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3txm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3txm OCA], [https://pdbe.org/3txm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3txm RCSB], [https://www.ebi.ac.uk/pdbsum/3txm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3txm ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3txm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3txm OCA], [https://pdbe.org/3txm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3txm RCSB], [https://www.ebi.ac.uk/pdbsum/3txm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3txm ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/PSD11_DROME PSD11_DROME]] Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, RPN6 is required for proteasome assembly (By similarity). May act as linker between 19S regulatory subunit and the 20S proteasome core.<ref>PMID:22187461</ref> 
[https://www.uniprot.org/uniprot/PSD11_DROME PSD11_DROME] Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, RPN6 is required for proteasome assembly (By similarity). May act as linker between 19S regulatory subunit and the 20S proteasome core.<ref>PMID:22187461</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Proteasomes execute the degradation of most cellular proteins. Although the 20S core particle (CP) has been studied in great detail, the structure of the 19S regulatory particle (RP), which prepares ubiquitylated substrates for degradation, has remained elusive. Here, we report the crystal structure of one of the RP subunits, Rpn6, and we describe its integration into the cryo-EM density map of the 26S holocomplex at 9.1 A resolution. Rpn6 consists of an alpha-solenoid-like fold and a proteasome COP9/signalosome eIF3 (PCI) module in a right-handed suprahelical configuration. Highly conserved surface areas of Rpn6 interact with the conserved surfaces of the Pre8 (alpha2) and Rpt6 subunits from the alpha and ATPase rings, respectively. The structure suggests that Rpn6 has a pivotal role in stabilizing the otherwise weak interaction between the CP and the RP.
 
The proteasomal subunit Rpn6 is a molecular clamp holding the core and regulatory subcomplexes together.,Pathare GR, Nagy I, Bohn S, Unverdorben P, Hubert A, Korner R, Nickell S, Lasker K, Sali A, Tamura T, Nishioka T, Forster F, Baumeister W, Bracher A Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):149-54. Epub 2011 Dec 20. PMID:22187461<ref>PMID:22187461</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3txm" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Drome]]
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Bracher, A]]
[[Category: Bracher A]]
[[Category: Pathare, G R]]
[[Category: Pathare GR]]
[[Category: 26 s proteasome]]
[[Category: Alpha solenoid]]
[[Category: Hydrolase]]
[[Category: Lid]]
[[Category: Pci domain]]
[[Category: Protein binding]]
[[Category: Regulatory particle]]

Latest revision as of 13:15, 1 March 2024

Crystal structure of Rpn6 from Drosophila melanogaster, Gd(3+) complexCrystal structure of Rpn6 from Drosophila melanogaster, Gd(3+) complex

Structural highlights

3txm is a 1 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PSD11_DROME Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, RPN6 is required for proteasome assembly (By similarity). May act as linker between 19S regulatory subunit and the 20S proteasome core.[1]

See Also

References

  1. ↑ Pathare GR, Nagy I, Bohn S, Unverdorben P, Hubert A, Korner R, Nickell S, Lasker K, Sali A, Tamura T, Nishioka T, Forster F, Baumeister W, Bracher A. The proteasomal subunit Rpn6 is a molecular clamp holding the core and regulatory subcomplexes together. Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):149-54. Epub 2011 Dec 20. PMID:22187461 doi:10.1073/pnas.1117648108

3txm, resolution 3.00Å

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