1qjb: Difference between revisions
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[[Image:1qjb.gif|left|200px]] | [[Image:1qjb.gif|left|200px]] | ||
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'''14-3-3 ZETA/PHOSPHOPEPTIDE COMPLEX (MODE 1)''' | '''14-3-3 ZETA/PHOSPHOPEPTIDE COMPLEX (MODE 1)''' | ||
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==About this Structure== | ==About this Structure== | ||
1QJB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry | 1QJB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=14ps 14ps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QJB OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Yaffe, M B.]] | [[Category: Yaffe, M B.]] | ||
[[Category: 14-3-3]] | [[Category: 14-3-3]] | ||
[[Category: | [[Category: Phosphopeptide]] | ||
[[Category: | [[Category: Signal transducti]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:20:37 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 06:20, 3 May 2008
14-3-3 ZETA/PHOSPHOPEPTIDE COMPLEX (MODE 1)
OverviewOverview
We have solved the high-resolution X-ray structure of 14-3-3 bound to two different phosphoserine peptides, representing alternative substrate-binding motifs. These structures reveal an evolutionarily conserved network of peptide-protein interactions within all 14-3-3 isotypes, explain both binding motifs, and identify a novel intrachain phosphorylation-mediated loop structure in one of the peptides. A 14-3-3 mutation disrupting Raf signaling alters the ligand-binding cleft, selecting a different phosphopeptide-binding motif and different substrates than the wild-type protein. Many 14-3-3: peptide contacts involve a C-terminal amphipathic alpha helix containing a putative nuclear export signal, implicating this segment in both ligand and Crm1 binding. Structural homology between the 14-3-3 NES structure and those within I kappa B alpha and p53 reveals a conserved topology recognized by the Crm1 nuclear export machinery.
About this StructureAbout this Structure
1QJB is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 14ps. Full crystallographic information is available from OCA.
ReferenceReference
Structural analysis of 14-3-3 phosphopeptide complexes identifies a dual role for the nuclear export signal of 14-3-3 in ligand binding., Rittinger K, Budman J, Xu J, Volinia S, Cantley LC, Smerdon SJ, Gamblin SJ, Yaffe MB, Mol Cell. 1999 Aug;4(2):153-66. PMID:10488331 Page seeded by OCA on Sat May 3 06:20:37 2008