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| ==Crystal structure of polyprenyl synthetase from Streptomyces coelicolor A3(2)== | | ==Crystal structure of polyprenyl synthetase from Streptomyces coelicolor A3(2)== |
| <StructureSection load='3nf2' size='340' side='right' caption='[[3nf2]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='3nf2' size='340' side='right'caption='[[3nf2]], [[Resolution|resolution]] 2.20Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3nf2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NF2 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3nf2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NF2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NF2 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SC6A5.12, SCO6763, Trans_IPPS_HT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nf2 OCA], [http://pdbe.org/3nf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nf2 RCSB], [http://www.ebi.ac.uk/pdbsum/3nf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nf2 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nf2 OCA], [https://pdbe.org/3nf2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nf2 RCSB], [https://www.ebi.ac.uk/pdbsum/3nf2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nf2 ProSAT]</span></td></tr> |
| </table> | | </table> |
| | == Function == |
| | [https://www.uniprot.org/uniprot/Q9X7V8_STRCO Q9X7V8_STRCO] |
| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nf2 ConSurf]. | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nf2 ConSurf]. |
| <div style="clear:both"></div> | | <div style="clear:both"></div> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The number of available protein sequences has increased exponentially with the advent of high-throughput genomic sequencing, creating a significant challenge for functional annotation. Here, we describe a large-scale study on assigning function to unknown members of the trans-polyprenyl transferase (E-PTS) subgroup in the isoprenoid synthase superfamily, which provides substrates for the biosynthesis of the more than 55,000 isoprenoid metabolites. Although the mechanism for determining the product chain length for these enzymes is known, there is no simple relationship between function and primary sequence, so that assigning function is challenging. We addressed this challenge through large-scale bioinformatics analysis of >5,000 putative polyprenyl transferases; experimental characterization of the chain-length specificity of 79 diverse members of this group; determination of 27 structures of 19 of these enzymes, including seven cocrystallized with substrate analogs or products; and the development and successful application of a computational approach to predict function that leverages available structural data through homology modeling and docking of possible products into the active site. The crystallographic structures and computational structural models of the enzyme-ligand complexes elucidate the structural basis of specificity. As a result of this study, the percentage of E-PTS sequences similar to functionally annotated ones (BLAST e-value </= 1e-70) increased from 40.6 to 68.8%, and the percentage of sequences similar to available crystal structures increased from 28.9 to 47.4%. The high accuracy of our blind prediction of newly characterized enzymes indicates the potential to predict function to the complete polyprenyl transferase subgroup of the isoprenoid synthase superfamily computationally.
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| Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily.,Wallrapp FH, Pan JJ, Ramamoorthy G, Almonacid DE, Hillerich BS, Seidel R, Patskovsky Y, Babbitt PC, Almo SC, Jacobson MP, Poulter CD Proc Natl Acad Sci U S A. 2013 Mar 14. PMID:23493556<ref>PMID:23493556</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3nf2" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Almo, S C]] | | [[Category: Large Structures]] |
| [[Category: Burley, S K]] | | [[Category: Streptomyces coelicolor]] |
| [[Category: Dickey, M]] | | [[Category: Almo SC]] |
| [[Category: Structural genomic]] | | [[Category: Burley SK]] |
| [[Category: NYSGXRC, New York SGX Research Center for Structural Genomics]] | | [[Category: Dickey M]] |
| [[Category: Patskovsky, Y]] | | [[Category: Patskovsky Y]] |
| [[Category: Sauder, J M]] | | [[Category: Sauder JM]] |
| [[Category: Toro, R]] | | [[Category: Toro R]] |
| [[Category: Isoprenyl diphosphate synthase]]
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| [[Category: Nysgrc]]
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| [[Category: PSI, Protein structure initiative]]
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| [[Category: Transferase]]
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