3lzo: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 ==Crystal Structure Analysis of the copper-reconstituted P19 protein from Campylobacter jejuni at 1.65 A at pH 10.0==
-<StructureSection load='3lzo' size='340' side='right' caption='[[3lzo]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
+<StructureSection load='3lzo' size='340' side='right'caption='[[3lzo]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3lzo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camjj Camjj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LZO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3lzo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni_81-176 Campylobacter jejuni subsp. jejuni 81-176]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LZO FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2o6c|2o6c]], [[2o6d|2o6d]], [[2o6e|2o6e]], [[2o6f|2o6f]], [[3lzp|3lzp]], [[3lzq|3lzq]], [[3lzl|3lzl]], [[3lzn|3lzn]], [[3lzr|3lzr]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CJJ81176_1650, Gene Cj81176_1659 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354242 CAMJJ])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lzo OCA], [https://pdbe.org/3lzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lzo RCSB], [https://www.ebi.ac.uk/pdbsum/3lzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lzo ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lzo OCA], [http://pdbe.org/3lzo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lzo RCSB], [http://www.ebi.ac.uk/pdbsum/3lzo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lzo ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0H3PA01_CAMJJ A0A0H3PA01_CAMJJ]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 19: / Line 20: @@
 </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lzo ConSurf].
 <div style="clear:both"></div>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Campylobacter jejuni, a major cause of acute bacterial diarrhea in humans, expresses numerous proteins to import diverse forms of essential iron. The expression of p19 and an adjacent iron transporter homologue (ftr1) is strongly induced upon iron limitation, suggesting a function in iron acquisition. Here, we show that the loss of P19 alone is detrimental to growth on iron-restricted media. Furthermore, metal binding analysis demonstrates that recombinant P19 has distinct copper and iron binding sites. Crystal structures of P19 have been solved to 1.41 A resolution, revealing an immunoglobulin-like fold. A P19 homodimer in which both monomers contribute ligands to two equivalent copper sites located adjacent to methionine-rich patches is observed. Copper coordination occurs via three histidine residues (His42, His95, and His132) and Met88. A solvent channel lined with conserved acidic residues leads to the copper site. Soaking crystals with a solution of manganese as iron analog reveals a second metal binding site in this solvent channel (metal-metal distance, 7.7 A). Glu44 lies between the metal sites and displays multiple conformations in the crystal structures, suggesting a role in regulating metal-metal interaction. Dimerization is shown to be metal dependent in vitro and is detected in vivo by cross-linking.
-Structure and function of P19, a high-affinity iron transporter of the human pathogen Campylobacter jejuni.,Chan AC, Doukov TI, Scofield M, Tom-Yew SA, Ramin AB, Mackichan JK, Gaynor EC, Murphy ME J Mol Biol. 2010 Aug 27;401(4):590-604. Epub 2010 Jun 30. PMID:20600116<ref>PMID:20600116</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 3lzo" style="background-color:#fffaf0;"></div>
 ==See Also==
 *[[P19|P19]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Camjj]]
+[[Category: Campylobacter jejuni subsp. jejuni 81-176]]
-[[Category: Chan, A C.K]]
+[[Category: Large Structures]]
-[[Category: Doukov, T I]]
+[[Category: Chan ACK]]
-[[Category: Murphy, M E.P]]
+[[Category: Doukov TI]]
-[[Category: Ramin, A B]]
+[[Category: Murphy MEP]]
-[[Category: Scofield, M]]
+[[Category: Ramin AB]]
-[[Category: Tom-Yew, S A.L]]
+[[Category: Scofield M]]
-[[Category: Copper binding]]
+[[Category: Tom-Yew SAL]]
-[[Category: Iron transport]]
-[[Category: Iron uptake]]
-[[Category: P19 delition]]
-[[Category: Transport protein]]