3dw7: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3dw7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DW7 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3dw7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DW7 FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1q9a|1q9a]], [[3dvz|3dvz]], [[3dw4|3dw4]], [[3dw5|3dw5]], [[3dw6|3dw6]], [[483d|483d]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dw7 OCA], [https://pdbe.org/3dw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dw7 RCSB], [https://www.ebi.ac.uk/pdbsum/3dw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dw7 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dw7 OCA], [https://pdbe.org/3dw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dw7 RCSB], [https://www.ebi.ac.uk/pdbsum/3dw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dw7 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Site-specific 2'-methylseleno RNA labeling is a promising tool for tackling the phase problem in RNA crystallography. We have developed an efficient strategy for crystallization and structure determination of RNA and RNA/protein complexes based on preliminary crystallization screening of 2'-OCH(3)-modified RNA sequences, prior to the replacement of 2'-OCH(3) groups with their 2'-SeCH(3) counterparts. The method exploits the similar crystallization properties of 2'-OCH(3)- and 2'-SeCH(3)-modified RNAs and has been successfully validated for two test cases. In addition, our data show that 2'-SeCH(3)-modified RNA have an increased resistance to X-ray radiolysis in comparison with commonly used 5-halogen-modified RNA, which permits collection of experimental electron density maps of remarkable quality.
A fast selenium derivatization strategy for crystallization and phasing of RNA structures.,Olieric V, Rieder U, Lang K, Serganov A, Schulze-Briese C, Micura R, Dumas P, Ennifar E RNA. 2009 Apr;15(4):707-15. Epub 2009 Feb 18. PMID:19228585<ref>PMID:19228585</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3dw7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Dumas, P]]
[[Category: Dumas P]]
[[Category: Ennifar, E]]
[[Category: Ennifar E]]
[[Category: Lang, K]]
[[Category: Lang K]]
[[Category: Micura, R]]
[[Category: Micura R]]
[[Category: Olieric, V]]
[[Category: Olieric V]]
[[Category: Rieder, U]]
[[Category: Rieder U]]
[[Category: Schulze-Briese, C]]
[[Category: Schulze-Briese C]]
[[Category: Serganov, A]]
[[Category: Serganov A]]
[[Category: Rna]]
[[Category: Sarcin ricin loop]]

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