1qfh: Difference between revisions

Revision as of 06:12, 3 May 2008

DIMERIZATION OF GELATION FACTOR FROM DICTYOSTELIUM DISCOIDEUM: CRYSTAL STRUCTURE OF ROD DOMAINS 5 AND 6

OverviewOverview

Gelation factor (ABP120) is one of the principal actin-cross-linking proteins of Dictyostelium discoideum. The extended molecule has an N-terminal 250-residue actin-binding domain and a rod constructed from six 100-residue repeats that have an Ig fold. The ability to dimerize is crucial to the actin cross-linking function of gelation factor and is mediated by the rod in which the two chains are arranged in an antiparallel fashion. We report the 2.2 A resolution crystal structure of rod domains 5 and 6, which shows that dimerization is mediated primarily by rod domain 6 and is the result of a double edge-to-edge extension of beta-sheets. Thus, contrary to earlier proposals, the chains of the dimeric gelation factor molecule overlap only within domain 6, and domains 1-5 do not pair with domains from the other chain. This information allows construction of a model of the gelation factor molecule and suggests how the chains in the related molecule filamin (ABP280) may interact.

About this StructureAbout this Structure

1QFH is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for dimerization of the Dictyostelium gelation factor (ABP120) rod., McCoy AJ, Fucini P, Noegel AA, Stewart M, Nat Struct Biol. 1999 Sep;6(9):836-41. PMID:10467095 Page seeded by OCA on Sat May 3 06:12:21 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1qfh.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1qfh |SIZE=350|CAPTION= <scene name='initialview01'>1qfh</scene>, resolution 2.2&Aring;
+The line below this paragraph, containing "STRUCTURE_1qfh", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1qfh|  PDB=1qfh  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qfh OCA], [http://www.ebi.ac.uk/pdbsum/1qfh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qfh RCSB]</span>
-}}
 '''DIMERIZATION OF GELATION FACTOR FROM DICTYOSTELIUM DISCOIDEUM: CRYSTAL STRUCTURE OF ROD DOMAINS 5 AND 6'''
@@ Line 29: / Line 26: @@
 [[Category: Noegel, A A.]]
 [[Category: Stewart, M.]]
-[[Category: abp-120]]
+[[Category: Abp-120]]
-[[Category: actin binding protein]]
+[[Category: Actin binding protein]]
-[[Category: gelation factor]]
+[[Category: Gelation factor]]
-[[Category: immunoglobulin]]
+[[Category: Immunoglobulin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:12:21 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:12:47 2008''