1qf6: Difference between revisions

Revision as of 06:11, 3 May 2008

STRUCTURE OF E. COLI THREONYL-TRNA SYNTHETASE COMPLEXED WITH ITS COGNATE TRNA

OverviewOverview

E. coli threonyl-tRNA synthetase (ThrRS) is a class II enzyme that represses the translation of its own mRNA. We report the crystal structure at 2.9 A resolution of the complex between tRNA(Thr) and ThrRS, whose structural features reveal novel strategies for providing specificity in tRNA selection. These include an amino-terminal domain containing a novel protein fold that makes minor groove contacts with the tRNA acceptor stem. The enzyme induces a large deformation of the anticodon loop, resulting in an interaction between two adjacent anticodon bases, which accounts for their prominent role in tRNA identity and translational regulation. A zinc ion found in the active site is implicated in amino acid recognition/discrimination.

About this StructureAbout this Structure

1QF6 is a Single protein structure of sequence from Escherichia coli. The following page contains interesting information on the relation of 1QF6 with [Aminoacyl-tRNA Synthetases]. Full crystallographic information is available from OCA.

ReferenceReference

The structure of threonyl-tRNA synthetase-tRNA(Thr) complex enlightens its repressor activity and reveals an essential zinc ion in the active site., Sankaranarayanan R, Dock-Bregeon AC, Romby P, Caillet J, Springer M, Rees B, Ehresmann C, Ehresmann B, Moras D, Cell. 1999 Apr 30;97(3):371-81. PMID:10319817 Page seeded by OCA on Sat May 3 06:11:36 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1qf6.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1qf6 |SIZE=350|CAPTION= <scene name='initialview01'>1qf6</scene>, resolution 2.9&Aring;
+The line below this paragraph, containing "STRUCTURE_1qf6", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=5MU:5-METHYLURIDINE+5&#39;-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=AET:N-[N-(9-B-D-RIBOFURANOSYLPURIN-6-YL)METHYLCARBAMOYL]THREONINE-5&#39;-MONOPHOSPHATE'>AET</scene>, <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=G7M:N7-METHYL-GUANOSINE-5&#39;-MONOPHOSPHATE'>G7M</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5&#39;-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5&#39;-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Threonine--tRNA_ligase Threonine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.3 6.1.1.3] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1qf6|  PDB=1qf6  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qf6 OCA], [http://www.ebi.ac.uk/pdbsum/1qf6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qf6 RCSB]</span>
-}}
 '''STRUCTURE OF E. COLI THREONYL-TRNA SYNTHETASE COMPLEXED WITH ITS COGNATE TRNA'''
@@ Line 31: / Line 28: @@
 [[Category: Rees, B.]]
 [[Category: Sankaranarayanan, R.]]
-[[Category: aminoacylation]]
+[[Category: Aminoacylation]]
-[[Category: amp]]
+[[Category: Amp]]
-[[Category: mrna]]
+[[Category: Mrna]]
-[[Category: protein/rna]]
+[[Category: Protein/rna]]
-[[Category: threonyl-trna synthetase]]
+[[Category: Threonyl-trna synthetase]]
-[[Category: translational regulation]]
+[[Category: Translational regulation]]
-[[Category: trna(thr)]]
+[[Category: Zinc]]
-[[Category: zinc]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:11:36 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:12:37 2008''