1qes: Difference between revisions
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'''TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES''' | '''TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QES OCA]. | |||
==Reference== | ==Reference== | ||
Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2., McDowell JA, He L, Chen X, Turner DH, Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9201950 9201950] | Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2., McDowell JA, He L, Chen X, Turner DH, Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9201950 9201950] | ||
[[Category: Chen, X.]] | [[Category: Chen, X.]] | ||
[[Category: He, L.]] | [[Category: He, L.]] | ||
[[Category: Mcdowell, J A.]] | [[Category: Mcdowell, J A.]] | ||
[[Category: Turner, D H.]] | [[Category: Turner, D H.]] | ||
[[Category: | [[Category: G:u mismatch]] | ||
[[Category: | [[Category: Ribonucleic acid]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:10:55 2008'' | |||
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Revision as of 06:10, 3 May 2008
TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES
OverviewOverview
The symmetric, tandem GU mismatch motifs, and , which only differ in the mismatch order, have an average difference in thermodynamic stability of 2 kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing these motifs indicate the effect is largely localized to the mismatches and adjacent base pairs. The three-dimensional structures of two representative duplexes, (rGGAGUUCC)2 and (rGGAUGUCC)2, were determined by two-dimensional NMR and a simulated annealing protocol. Local deviations are similar to other intrahelical GU mismatches with little effect on backbone torsion angles and a slight overtwisting between the base pair 5' of the G of the mismatch and the mismatch itself. Comparisons of the resulting stacking patterns along with electrostatic potential maps suggest that interactions between highly negative electrostatic regions between base pairs may play a role in the observed thermodynamic differences.
About this StructureAbout this Structure
Full crystallographic information is available from OCA.
ReferenceReference
Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2., McDowell JA, He L, Chen X, Turner DH, Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:9201950 Page seeded by OCA on Sat May 3 06:10:55 2008