Proteopedia

2lkf: Difference between revisions

← Older edit
No edit summary
No edit summary
 
Line 3: Line 3:
<StructureSection load='2lkf' size='340' side='right'caption='[[2lkf]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='2lkf' size='340' side='right'caption='[[2lkf]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2lkf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LKF FirstGlance]. <br>
<table><tr><td colspan='2'>[[2lkf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LKF FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LUKF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lkf OCA], [https://pdbe.org/2lkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lkf RCSB], [https://www.ebi.ac.uk/pdbsum/2lkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lkf ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lkf OCA], [https://pdbe.org/2lkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lkf RCSB], [https://www.ebi.ac.uk/pdbsum/2lkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lkf ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/HLGB_STAAU HLGB_STAAU]] Toxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity.  
[https://www.uniprot.org/uniprot/HLGB_STAAU HLGB_STAAU] Toxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Line 19: Line 19:
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2lkf ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2lkf ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are self-assembling, channel-forming proteins related in sequence and function. In the alpha-hemolysin heptamer, the channel-forming beta-strands and the amino latch make long excursions from the protomer core. Here we report the crystal structure of the water soluble form of LukF. In the LukF structure the channel-forming region folds into an amphipathic, three-strand beta-sheet and the amino latch forms a beta-strand extending a central beta-sheet. The LukF structure illustrates how a channel-forming toxin masks protein-protein and protein-membrane interfaces prior to cell binding and assembly, and together with the alpha-hemolysin heptamer structure, they define the end points on the pathway of toxin assembly.
Crystal structure of staphylococcal LukF delineates conformational changes accompanying formation of a transmembrane channel.,Olson R, Nariya H, Yokota K, Kamio Y, Gouaux E Nat Struct Biol. 1999 Feb;6(2):134-40. PMID:10048924<ref>PMID:10048924</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2lkf" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Pore forming toxin%2C α-hemolysin|Pore forming toxin%2C α-hemolysin]]
*[[Pore forming toxin%2C α-hemolysin|Pore forming toxin%2C α-hemolysin]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Gouaux, J E]]
[[Category: Staphylococcus aureus]]
[[Category: Kamio, Y]]
[[Category: Gouaux JE]]
[[Category: Nariya, H]]
[[Category: Kamio Y]]
[[Category: Olson, R]]
[[Category: Nariya H]]
[[Category: Yokota, K]]
[[Category: Olson R]]
[[Category: Hemolysin]]
[[Category: Yokota K]]
[[Category: Leukotoxin]]
[[Category: Pore-forming toxin]]
[[Category: Toxin]]

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/w/2lkf"