1qdl: Difference between revisions

Revision as of 06:09, 3 May 2008

THE CRYSTAL STRUCTURE OF ANTHRANILATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS

OverviewOverview

Anthranilate synthase catalyzes the synthesis of anthranilate from chorismate and glutamine and is feedback-inhibited by tryptophan. The enzyme of the hyperthermophile Sulfolobus solfataricus has been crystallized in the absence of physiological ligands, and its three-dimensional structure has been determined at 2.5-A resolution with x-ray crystallography. It is a heterotetramer of anthranilate synthase (TrpE) and glutamine amidotransferase (TrpG) subunits, in which two TrpG:TrpE protomers associate mainly via the TrpG subunits. The small TrpG subunit (195 residues) has the known "triad" glutamine amidotransferase fold. The large TrpE subunit (421 residues) has a novel fold. It displays a cleft between two domains, the tips of which contact the TrpG subunit across its active site. Clusters of catalytically essential residues are located inside the cleft, spatially separated from clustered residues involved in feedback inhibition. The structure suggests a model in which chorismate binding triggers a relative movement of the two domain tips of the TrpE subunit, activating the TrpG subunit and creating a channel for passage of ammonia toward the active site of the TrpE subunit. Tryptophan presumably blocks this rearrangement, thus stabilizing the inactive states of both subunits. The structure of the TrpE subunit is a likely prototype for the related enzymes 4-amino 4-deoxychorismate synthase and isochorismate synthase.

About this StructureAbout this Structure

1QDL is a Protein complex structure of sequences from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications., Knochel T, Ivens A, Hester G, Gonzalez A, Bauerle R, Wilmanns M, Kirschner K, Jansonius JN, Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9479-84. PMID:10449718 Page seeded by OCA on Sat May 3 06:09:48 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1qdl.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1qdl |SIZE=350|CAPTION= <scene name='initialview01'>1qdl</scene>, resolution 2.500&Aring;
+The line below this paragraph, containing "STRUCTURE_1qdl", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1qdl|  PDB=1qdl  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qdl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qdl OCA], [http://www.ebi.ac.uk/pdbsum/1qdl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qdl RCSB]</span>
-}}
 '''THE CRYSTAL STRUCTURE OF ANTHRANILATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS'''
@@ Line 34: / Line 31: @@
 [[Category: Knoechel, T.]]
 [[Category: Wilmanns, M.]]
-[[Category: allosteric interaction]]
+[[Category: Allosteric interaction]]
-[[Category: anthranilate synthase]]
+[[Category: Anthranilate synthase]]
-[[Category: glutamine amidotransferase]]
+[[Category: Glutamine amidotransferase]]
-[[Category: tryptophan biosynthesis]]
+[[Category: Tryptophan biosynthesis]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:09:48 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:12:15 2008''