1qci: Difference between revisions

Revision as of 06:07, 3 May 2008

LOW TEMPERATURE STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN COMPLEXED WITH ADENINE

OverviewOverview

The pokeweed antiviral protein (PAP) belongs to a family of ribosome-inactivating proteins (RIP), which depurinate ribosomal RNA through their site-specific N-glycosidase activity. We report low temperature, three-dimensional structures of PAP co-crystallized with adenyl-guanosine (ApG) and adenyl-cytosine-cytosine (ApCpC). Crystal structures of 2.0-2.1 A resolution revealed that both ApG or ApCpC nucleotides are cleaved by PAP, leaving only the adenine base clearly visible in the active site pocket of PAP. ApCpC does not resemble any known natural substrate for any ribosome-inactivating proteins and its cleavage by PAP provides unprecedented evidence for a broad spectrum N-glycosidase activity of PAP toward adenine-containing single stranded RNA. We also report the analysis of a 2.1 A crystal structure of PAP complexed with the RIP inhibitor pteoric acid. The pterin ring is strongly bound in the active site, forming four hydrogen bonds with active site residues and one hydrogen bond with the coordinated water molecule. The second 180 degrees rotation conformation of pterin ring can form only three hydrogen bonds in the active site and is less energetically favorable. The benzoate moiety is parallel to the protein surface of PAP and forms only one hydrogen bond with the guanido group of Arg135.

About this StructureAbout this Structure

1QCI is a Single protein structure of sequence from Phytolacca americana. Full crystallographic information is available from OCA.

ReferenceReference

X-ray crystallographic analysis of the structural basis for the interactions of pokeweed antiviral protein with its active site inhibitor and ribosomal RNA substrate analogs., Kurinov IV, Myers DE, Irvin JD, Uckun FM, Protein Sci. 1999 Sep;8(9):1765-72. PMID:10493577 Page seeded by OCA on Sat May 3 06:07:50 2008

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OCA

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 [[Image:1qci.gif|left|200px]]
- {{Structure
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-|RELATEDENTRY=[[1qcg|1QCG]], [[1qcj|1QCJ]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qci OCA], [http://www.ebi.ac.uk/pdbsum/1qci PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qci RCSB]</span>
-}}
 '''LOW TEMPERATURE STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN COMPLEXED WITH ADENINE'''
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 [[Category: Myers, D E.]]
 [[Category: Uckun, F M.]]
-[[Category: pokeweed antiviral protein]]
+[[Category: Pokeweed antiviral protein]]
-[[Category: ribosome inactivating protein]]
+[[Category: Ribosome inactivating protein]]
-[[Category: rna substrate analog]]
+[[Category: Rna substrate analog]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:07:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:11:48 2008''