7uy7: Difference between revisions

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 == Structural highlights ==
 <table><tr><td colspan='2'>[[7uy7]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UY7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UY7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uy7 OCA], [https://pdbe.org/7uy7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uy7 RCSB], [https://www.ebi.ac.uk/pdbsum/7uy7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uy7 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/TAP19_TETTS TAP19_TETTS]] Component of a CST-like subcomplex of the holoenzyme telomerase ribonucleoprotein complex, which stimulates telomerase complementary-strand synthesis (PubMed:26551074). Telomerase is an essential ribonucleoprotein enzyme that copies new telomeric repeats onto chromosome ends by repetitively synthesizing the short telomere-repeat sequence 5'-TTGGGG-3' using an RNA template component TER (PubMed:26551074). The CST-like subcomplex (also named 7-4-1) binds telomeric single-stranded DNA and coordinates telomere G-strand and C-strand synthesis (PubMed:26551074).<ref>PMID:26551074</ref>
+[https://www.uniprot.org/uniprot/TAP19_TETTS TAP19_TETTS] Component of a CST-like subcomplex of the holoenzyme telomerase ribonucleoprotein complex, which stimulates telomerase complementary-strand synthesis (PubMed:26551074). Telomerase is an essential ribonucleoprotein enzyme that copies new telomeric repeats onto chromosome ends by repetitively synthesizing the short telomere-repeat sequence 5'-TTGGGG-3' using an RNA template component TER (PubMed:26551074). The CST-like subcomplex (also named 7-4-1) binds telomeric single-stranded DNA and coordinates telomere G-strand and C-strand synthesis (PubMed:26551074).<ref>PMID:26551074</ref>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Telomeres are the physical ends of linear chromosomes. They are composed of short repeating sequences (such as TTGGGG in the G-strand for Tetrahymena thermophila) of double-stranded DNA with a single-strand 3' overhang of the G-strand and, in humans, the six shelterin proteins: TPP1, POT1, TRF1, TRF2, RAP1 and TIN2(1,2). TPP1 and POT1 associate with the 3' overhang, with POT1 binding the G-strand(3) and TPP1 (in complex with TIN2(4)) recruiting telomerase via interaction with telomerase reverse transcriptase(5) (TERT). The telomere DNA ends are replicated and maintained by telomerase(6), for the G-strand, and subsequently DNA polymerase alpha-primase(7,8) (PolalphaPrim), for the C-strand(9). PolalphaPrim activity is stimulated by the heterotrimeric complex CTC1-STN1-TEN1(10-12) (CST), but the structural basis of the recruitment of PolalphaPrim and CST to telomere ends remains unknown. Here we report cryo-electron microscopy (cryo-EM) structures of Tetrahymena CST in the context of the telomerase holoenzyme, in both the absence and the presence of PolalphaPrim, and of PolalphaPrim alone. Tetrahymena Ctc1 binds telomerase subunit p50, a TPP1 orthologue, on a flexible Ctc1 binding motif revealed by cryo-EM and NMR spectroscopy. The PolalphaPrim polymerase subunit POLA1 binds Ctc1 and Stn1, and its interface with Ctc1 forms an entry port for G-strand DNA to the POLA1 active site. We thus provide a snapshot of four key components that are required for telomeric DNA synthesis in a single active complex-telomerase-core ribonucleoprotein, p50, CST and PolalphaPrim-that provides insights into the recruitment of CST and PolalphaPrim and the handoff between G-strand and C-strand synthesis.
-Structure of Tetrahymena telomerase-bound CST with polymerase alpha-primase.,He Y, Song H, Chan H, Liu B, Wang Y, Susac L, Zhou ZH, Feigon J Nature. 2022 Aug;608(7924):813-818. doi: 10.1038/s41586-022-04931-7. Epub 2022, Jul 13. PMID:35831498<ref>PMID:35831498</ref>
+==See Also==
+*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+*[[Telomerase 3D structures|Telomerase 3D structures]]
-</div>
-<div class="pdbe-citations 7uy7" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>