1q16: Difference between revisions

Revision as of 05:44, 3 May 2008

Crystal structure of Nitrate Reductase A, NarGHI, from Escherichia coli

OverviewOverview

The facultative anaerobe Escherichia coli is able to assemble specific respiratory chains by synthesis of appropriate dehydrogenases and reductases in response to the availability of specific substrates. Under anaerobic conditions in the presence of nitrate, E. coli synthesizes the cytoplasmic membrane-bound quinol-nitrate oxidoreductase (nitrate reductase A; NarGHI), which reduces nitrate to nitrite and forms part of a redox loop generating a proton-motive force. We present here the crystal structure of NarGHI at a resolution of 1.9 A. The NarGHI structure identifies the number, coordination scheme and environment of the redox-active prosthetic groups, a unique coordination of the molybdenum atom, the first structural evidence for the role of an open bicyclic form of the molybdo-bis(molybdopterin guanine dinucleotide) (Mo-bisMGD) cofactor in the catalytic mechanism and a novel fold of the membrane anchor subunit. Our findings provide fundamental molecular details for understanding the mechanism of proton-motive force generation by a redox loop.

About this StructureAbout this Structure

1Q16 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A., Bertero MG, Rothery RA, Palak M, Hou C, Lim D, Blasco F, Weiner JH, Strynadka NC, Nat Struct Biol. 2003 Sep;10(9):681-7. Epub 2003 Aug 10. PMID:12910261 Page seeded by OCA on Sat May 3 05:44:23 2008

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OCA

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 [[Image:1q16.gif|left|200px]]
- {{Structure
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrate_reductase Nitrate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.4 1.7.99.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= narG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), narH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), narI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q16 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q16 OCA], [http://www.ebi.ac.uk/pdbsum/1q16 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1q16 RCSB]</span>
-}}
 '''Crystal structure of Nitrate Reductase A, NarGHI, from Escherichia coli'''
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 [[Category: Bertero, M G.]]
 [[Category: Strynadka, N C.J.]]
-[[Category: electron-transfer]]
+[[Category: Electron-transfer]]
-[[Category: membrane protein]]
+[[Category: Membrane protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:44:23 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:07:24 2008''