1q10: Difference between revisions
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{{STRUCTURE_1q10| PDB=1q10 | SCENE= }} | |||
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'''Ensemble of 40 Structures of the Dimeric Mutant of the B1 Domain of Streptococcal Protein G''' | '''Ensemble of 40 Structures of the Dimeric Mutant of the B1 Domain of Streptococcal Protein G''' | ||
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[[Category: Gronenborn, A M.]] | [[Category: Gronenborn, A M.]] | ||
[[Category: Louis, J M.]] | [[Category: Louis, J M.]] | ||
[[Category: | [[Category: Core mutant]] | ||
[[Category: | [[Category: Domain-swapping]] | ||
[[Category: | [[Category: Gb1]] | ||
[[Category: | [[Category: Nmr structure]] | ||
[[Category: | [[Category: Oligomerization]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:44:03 2008'' | |||
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Revision as of 05:44, 3 May 2008
Ensemble of 40 Structures of the Dimeric Mutant of the B1 Domain of Streptococcal Protein G
OverviewOverview
Immunoglobulin-binding domain B1 of streptococcal protein G (GB1), a small (56 residues), stable, single-domain protein, is one of the most extensively used model systems in the area of protein folding and design. Recently, NMR and X-ray structures of a quintuple GB1 core mutant (L5V/A26F/F30V/Y33F/A34F) that showed an unexpected, intertwined tetrameric architecture were determined. Here, we report the NMR structure of another mutant, derived from the tetramer by reverting the single amino acid position F26 back to the wild-type sequence A26. The structure reveals a domain-swapped dimer that involves exchange of the second beta-hairpin. The resulting overall structure comprises an eight-stranded beta-sheet whose concave side is covered by two alpha helices. The dimer dissociates into a partially folded, monomeric species with a dissociation constant of 93(+/-10)microM.
About this StructureAbout this Structure
1Q10 is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.
ReferenceReference
A protein contortionist: core mutations of GB1 that induce dimerization and domain swapping., Byeon IJ, Louis JM, Gronenborn AM, J Mol Biol. 2003 Oct 10;333(1):141-52. PMID:14516749 Page seeded by OCA on Sat May 3 05:44:03 2008