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| <StructureSection load='1ehw' size='340' side='right'caption='[[1ehw]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='1ehw' size='340' side='right'caption='[[1ehw]], [[Resolution|resolution]] 2.40Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[1ehw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EHW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1EHW FirstGlance]. <br> | | <table><tr><td colspan='2'>[[1ehw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EHW FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] </span></td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ehw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ehw OCA], [http://pdbe.org/1ehw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ehw RCSB], [http://www.ebi.ac.uk/pdbsum/1ehw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ehw ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ehw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ehw OCA], [https://pdbe.org/1ehw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ehw RCSB], [https://www.ebi.ac.uk/pdbsum/1ehw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ehw ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/NDKM_HUMAN NDKM_HUMAN]] Major role in the synthesis of nucleoside triphosphates other than ATP (By similarity). | | [https://www.uniprot.org/uniprot/NDKM_HUMAN NDKM_HUMAN] Major role in the synthesis of nucleoside triphosphates other than ATP (By similarity). |
| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ehw ConSurf]. | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ehw ConSurf]. |
| <div style="clear:both"></div> | | <div style="clear:both"></div> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| We demonstrate here the catalytic activity and subcellular localization of the Nm23-H4 protein, product of nm23-H4, a new member of the human nm23/nucleoside diphosphate (NDP) kinase gene family (Milon, L., Rousseau-Merck, M., Munier, A., Erent, M., Lascu, I., Capeau, J., and Lacombe, M. L. (1997) Hum. Genet. 99, 550-557). Nm3-H4 was synthesized in escherichia coli as the full-length protein and as a truncated form missing the N-terminal extension characteristic of mitochondrial targeting. The truncated form possesses NDP kinase activity, whereas the full-length protein is inactive, suggesting that the extension prevents enzyme folding and/or activity. X-ray crystallographic analysis was performed on active truncated Nm23-H4. Like other eukaryotic NDP kinases, it is a hexamer. Nm23-H4 naturally possesses a serine residue at position 129, equivalent to the K-pn mutation of the Drosophila NDP kinase. The x-ray structure shows that the presence of Ser(129) has local structural effects that weaken subunit interactions. Site-directed mutagenesis shows that the serine is responsible for the lability of Nm23-H4 to heat and urea treatment, because the S129P mutant is greatly stabilized. Examination of human embryonic kidney 293 cells transfected with green fluorescent protein fusions by confocal microscopy shows a specific mitochondrial localization of Nm23-H4 that was also demonstrated by Western blot analysis of subcellular fractions of these cells. Import into mitochondria is accompanied by cleavage of the N-terminal extension that results in NDP kinase activity. Submitochondrial fractionation indicates that Nm23-H4 is associated with mitochondrial membranes, possibly to the contact sites between the outer and inner membranes.
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| The human nm23-H4 gene product is a mitochondrial nucleoside diphosphate kinase.,Milon L, Meyer P, Chiadmi M, Munier A, Johansson M, Karlsson A, Lascu I, Capeau J, Janin J, Lacombe ML J Biol Chem. 2000 May 12;275(19):14264-72. PMID:10799505<ref>PMID:10799505</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 1ehw" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Nucleoside diphosphate kinase 3D structures|Nucleoside diphosphate kinase 3D structures]] | | *[[Nucleoside diphosphate kinase 3D structures|Nucleoside diphosphate kinase 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Human]] | | [[Category: Homo sapiens]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Nucleoside-diphosphate kinase]]
| | [[Category: Capeau J]] |
| [[Category: Capeau, J]] | | [[Category: Chiadmi M]] |
| [[Category: Chiadmi, M]] | | [[Category: Janin J]] |
| [[Category: Janin, J]] | | [[Category: Johansson M]] |
| [[Category: Johansson, M]] | | [[Category: Karlsson A]] |
| [[Category: Karlsson, A]] | | [[Category: Lacombe M-L]] |
| [[Category: Lacombe, M L]] | | [[Category: Lascu I]] |
| [[Category: Lascu, I]] | | [[Category: Meyer P]] |
| [[Category: Meyer, P]] | | [[Category: Milon L]] |
| [[Category: Milon, L]] | | [[Category: Munier A]] |
| [[Category: Munier, A]] | |
| [[Category: Killer-of-prune]]
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| [[Category: Mitochondrial]]
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| [[Category: Nm23]]
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| [[Category: Nucleoside diphosphate kinase]]
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| [[Category: Transferase]]
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