1q0s: Difference between revisions

Revision as of 05:43, 3 May 2008

Binary Structure of T4DAM with AdoHcy

OverviewOverview

DNA-adenine methylation at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as bacterial virulence. We report here the crystal structures of the bacteriophage T4Dam DNA adenine methyltransferase (MTase) in a binary complex with the methyl-donor product S-adenosyl-L-homocysteine (AdoHcy) and in a ternary complex with a synthetic 12-bp DNA duplex and AdoHcy. T4Dam contains two domains: a seven-stranded catalytic domain that harbors the binding site for AdoHcy and a DNA binding domain consisting of a five-helix bundle and a beta-hairpin that is conserved in the family of GATC-related MTase orthologs. Unexpectedly, the sequence-specific T4Dam bound to DNA in a nonspecific mode that contained two Dam monomers per synthetic duplex, even though the DNA contains a single GATC site. The ternary structure provides a rare snapshot of an enzyme poised for linear diffusion along the DNA.

About this StructureAbout this Structure

1Q0S is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the bacteriophage T4 DNA adenine methyltransferase., Yang Z, Horton JR, Zhou L, Zhang XJ, Dong A, Zhang X, Schlagman SL, Kossykh V, Hattman S, Cheng X, Nat Struct Biol. 2003 Oct;10(10):849-55. Epub 2003 Aug 24. PMID:12937411 Page seeded by OCA on Sat May 3 05:43:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1q0s.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1q0s |SIZE=350|CAPTION= <scene name='initialview01'>1q0s</scene>, resolution 2.3&Aring;
+The line below this paragraph, containing "STRUCTURE_1q0s", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= DAM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])
+-->
-|DOMAIN=
+{{STRUCTURE_1q0s|  PDB=1q0s  |  SCENE=  }}
-|RELATEDENTRY=[[1q0t|1Q0T]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q0s OCA], [http://www.ebi.ac.uk/pdbsum/1q0s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1q0s RCSB]</span>
-}}
 '''Binary Structure of T4DAM with AdoHcy'''
@@ Line 25: / Line 22: @@
 [[Category: Enterobacteria phage t4]]
 [[Category: Single protein]]
-[[Category: Site-specific DNA-methyltransferase (adenine-specific)]]
 [[Category: Cheng, X.]]
 [[Category: Dong, A.]]
@@ Line 36: / Line 32: @@
 [[Category: Zhang, X J.]]
 [[Category: Zhou, L.]]
-[[Category: methyltransferase]]
+[[Category: Methyltransferase]]
-[[Category: t4dam]]
+[[Category: T4dam]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:43:39 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:07:16 2008''