1dn5: Difference between revisions

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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dn5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dn5 OCA], [https://pdbe.org/1dn5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dn5 RCSB], [https://www.ebi.ac.uk/pdbsum/1dn5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dn5 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dn5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dn5 OCA], [https://pdbe.org/1dn5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dn5 RCSB], [https://www.ebi.ac.uk/pdbsum/1dn5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dn5 ProSAT]</span></td></tr>
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== Publication Abstract from PubMed ==
Crystals of the hexadeoxyoligomer d(5BrC-G-5BrC-G-5BrC-G) were grown at different temperatures (5 degrees C, 18 degrees C and 37 degrees C) in the absence of divalent cations. The crystals grown at 5 degrees C did not diffract X-rays, while those grown at 18 degrees C and 37 degrees C did. The oligomer adopts the left-handed ZI conformation in both crystals. The main difference resides in a more extensive hydration shell in the crystal grown at high temperature than in the crystal grown at low temperature. The high-temperature crystal displays a spine of hydration running deep in the minor groove and linking exocyclic O-2 atoms of the pyrimidine rings. In both crystalline forms, a hydrated sodium ion bound to the N-7 of a guanine ring was found. Strings of water molecules bridging phosphate anionic oxygen atoms are found along the backbone. The absolute values of the propeller-twist are also different in both structures although the values of the twist are very similar. The results point to the importance of the crystallization conditions when analysing fine structural details like solvation properties of oligomers.
Solvation of the left-handed hexamer d(5BrC-G-5BrC-G-5 BrC-G) in crystals grown at two temperatures.,Chevrier B, Dock AC, Hartmann B, Leng M, Moras D, Thuong MT, Westhof E J Mol Biol. 1986 Apr 20;188(4):707-19. PMID:3735433<ref>PMID:3735433</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1dn5" style="background-color:#fffaf0;"></div>
== References ==
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Latest revision as of 09:56, 7 February 2024

SOLVATION OF THE LEFT-HANDED HEXAMER D(5BRC-G-5BRC-G-5BRC-G) IN CRYSTALS GROWN AT TWO TEMPERATURESSOLVATION OF THE LEFT-HANDED HEXAMER D(5BRC-G-5BRC-G-5BRC-G) IN CRYSTALS GROWN AT TWO TEMPERATURES

Structural highlights

1dn5 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.4Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

1dn5, resolution 1.40Å

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