1q07: Difference between revisions

Revision as of 05:42, 3 May 2008

Crystal structure of the Au(I) form of E. coli CueR, a copper efflux regulator

OverviewOverview

The earliest of a series of copper efflux genes in Escherichia coli are controlled by CueR, a member of the MerR family of transcriptional activators. Thermodynamic calibration of CueR reveals a zeptomolar (10(-21) molar) sensitivity to free Cu+, which is far less than one atom per cell. Atomic details of this extraordinary sensitivity and selectivity for +1transition-metal ions are revealed by comparing the crystal structures of CueR and a Zn2+-sensing homolog, ZntR. An unusual buried metal-receptor site in CueR restricts the metal to a linear, two-coordinate geometry and uses helix-dipole and hydrogen-bonding interactions to enhance metal binding. This binding mode is rare among metalloproteins but well suited for an ultrasensitive genetic switch.

About this StructureAbout this Structure

1Q07 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR., Changela A, Chen K, Xue Y, Holschen J, Outten CE, O'Halloran TV, Mondragon A, Science. 2003 Sep 5;301(5638):1383-7. PMID:12958362 Page seeded by OCA on Sat May 3 05:42:27 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1q07.gif|left|200px]]
- {{Structure
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-|GENE= CUER ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-|RELATEDENTRY=[[1q05|1Q05]], [[1q06|1Q06]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q07 OCA], [http://www.ebi.ac.uk/pdbsum/1q07 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1q07 RCSB]</span>
-}}
 '''Crystal structure of the Au(I) form of E. coli CueR, a copper efflux regulator'''
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 [[Category: Outten, C E.]]
 [[Category: Xue, Y.]]
-[[Category: copper efflux regulator]]
+[[Category: Copper efflux regulator]]
-[[Category: merr family transcriptional regulator]]
+[[Category: Merr family transcriptional regulator]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:42:27 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:07:07 2008''